A newly established canine NK-type cell line and its cytotoxic properties

被引：12

作者：

Grudzien, Malgorzata ^{[1
]}

Pawlak, Aleksandra ^{[2
]}

Kutkowska, Justyna ^{[1
]}

Ziolo, Ewa ^{[1
]}

Wysokinska, Edyta ^{[1
]}

Hildebrand, Wojciech ^{[3
]}

Obminska-Mrukowicz, Bolena ^{[2
]}

Strzadala, Leon ^{[1
]}

Rapak, Andrzej ^{[1
]}

机构：

[1] Ludw Hirszfeld Inst Immunol & Expt Therapy, Lab Tumor Mol Immunobiol, Wroclaw, Poland

[2] Wroclaw Univ Environm & Life Sci, Dept Biochem Pharmacol & Toxicol, Wroclaw, Poland

[3] Vet Clin NeoVet Wroclaw, Wroclaw, Poland

来源：

VETERINARY AND COMPARATIVE ONCOLOGY | 2021年 / 19卷 / 03期

关键词：

immunology; innate immunity; lymphocytes; natural killer cells; oncology; small animal;

D O I：

10.1111/vco.12695

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

We established a canine natural killer (NK)-type cell line called CNK-89 derived from a dog with NK cell neoplasia. Immunophenotyping analysis showed positive staining for CD5, CD8, CD45, CD56, CD79a and NKp46, while negative for CD3, CD4, CD14, CD20, CD21, CD34, Thy1, IgG, IgM and MHCII. Polymerase chain reaction analysis revealed the presence of CD56, NKG2D, NKp30, NKp44, NKp46 and perforin, but the absence of CD16, Ly49 and granzyme B mRNA. Treating CNK-89 cells with IL-2 did not change the expression of activating receptors, TNF alpha and IFN gamma secretion and cytotoxic activity, however, treatment with IL-12 alone or in combinations with IL-15, IL-18 and IL-21 caused an increase in granzyme B and CD16 mRNA, IFN gamma secretion and cytotoxic properties of the CNK-89 cell line.

引用

页码：567 / 577

页数：11

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