Rifabutin and rifampin resistance levels and associated rpoB mutations in clinical isolates of Mycobacterium tuberculosis complex

被引：57

作者：

Berrada, Zenda L. ^{[1
,3
]}

Lin, Shou-Yean Grace ^{[1
]}

Rodwell, Timothy C. ^{[2
]}

Duylinh Nguyen ^{[1
,4
]}

Schecter, Gisela F. ^{[1
]}

Pham, Lucy ^{[1
]}

Janda, J. Michael ^{[1
,5
]}

Elmaraachli, Wael ^{[2
]}

Catanzaro, Antonino ^{[2
]}

Desmond, Edward ^{[1
]}

机构：

[1] Calif Dept Publ Hlth, Microbial Dis Lab, Richmond, CA USA

[2] Univ Calif San Diego, San Diego, CA 92103 USA

[3] San Mateo Cty Dept, Hlth Publ Hlth Lab, San Mateo, CA USA

[4] Roche Mol Diagnost, Pleasanton, CA USA

[5] Kern Cty Hlth Dept, Publ Hlth Lab, Bakersfield, CA USA

来源：

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2016年 / 85卷 / 02期

关键词：

Mycobacterium tuberculosis; multi-drug resistant tuberculosis; rifabutin; molecular drug-susceptibility; MULTICENTER LABORATORY VALIDATION; IN-VITRO ACTIVITY; CROSS-RESISTANCE; DRUG-RESISTANCE; MOLECULAR DIAGNOSIS; CLASSICAL; 2ND-LINE; RAPID DETECTION; STRAINS; SUSCEPTIBILITY; GENE;

D O I：

10.1016/j.diagmicrobio.2016.01.019

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Cross-resistance in rifamycins has been observed in rifampin (RIF)-resistant Mycobacterium tuberculosis complex isolates; some rpoB mutations do not confer broad in vitro rifamycin resistance. We examined 164 isolates, of which 102 were RIF-resistant, for differential resistance between RIF and rifabutin (RFB). A total of 42 unique single mutations or combinations of mutations were detected. The number of unique mutations identified exceeded that reported in any previous study. RFB and RIF MICs up to 8 mu g/mL by MGIT 960 were studied; the cut-off values for susceptibility to RIF and RFB were 1 mu g/mL and 0.5 mu g/mL, respectively. We identified 31 isolates resistant to RIF but susceptible to RFB with the mutations D516V, D516F, 518 deletion, S522L, H526A, H526C, H526G, H526L, and two dual mutations (S522L + K527R and H526S + K527R). Clinical investigations using RFB to treat multidrug-resistant tuberculosis cases harboring those mutations are recommended. (C) 2016 The Authors. Published by Elsevier Inc.

引用

页码：177 / 181

页数：5

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