Cpf1 proteins excise CRISPR RNAs from mRNA transcripts in mammalian cells

被引：57

作者：

Zhong, Guocai ^{[1
]}

Wang, Haimin ^{[1
]}

Li, Yujun ^{[1
,2
,3
]}

Tran, Mai H. ^{[1
]}

Farzan, Michael ^{[1
]}

机构：

[1] Scripps Res Inst, Dept Immunol & Microbiol, Jupiter, FL 33458 USA

[2] Harbin Med Univ, Dept Microbiol, Harbin, Peoples R China

[3] Harbin Med Univ, Wu Lien Teh Inst, Harbin, Peoples R China

来源：

NATURE CHEMICAL BIOLOGY | 2017年 / 13卷 / 08期

关键词：

SPECIFICITIES;

D O I：

10.1038/nchembio.2410

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cpf1 is a CRISPR effector protein that has greater specificity than Streptococcus pyogenes Cas9 (SpCas9) in genome-editing applications. Here we show that Lachnospiraceae bacterium (Lb) and Acidaminococus sp. (As) Cpf1 orthologs have RNase activities that can excise multiple CRISPR RNAs (crRNAs) from a single RNA polymerase II-driven RNA transcript expressed in mammalian cells. This property simplifies modification of multiple genomic targets and can be used to increase the efficiency of Cpf1-mediated editing.

引用

页码：839 / +

页数：5

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