A novel polysaccharide from Malus halliana Koehne with coagulant activity

被引：17

作者：

Zhang, Xiaofeng ^{[1
]}

Yu, Qi ^{[1
]}

Jiang, Huimin ^{[1
]}

Ma, Changyang ^{[1
,2
]}

Wang, Hui Min David ^{[3
]}

Wang, Jinmei ^{[1
,2
]}

Kang, Wen-Yi ^{[1
,2
]}

机构：

[1] Henan Univ, Natl R&D Ctr Edible Fungus Proc Technol, Kaifeng 475004, Peoples R China

[2] Kaifeng Key Lab Funct Components Hlth Food, Kaifeng 475004, Peoples R China

[3] Natl Chung Hsing Univ, Grad Inst Biomed Engn, Taichung 402, Taiwan

来源：

CARBOHYDRATE RESEARCH | 2019年 / 485卷

关键词：

Malus halliana Koehne; Polysaccharides; Structural characterization; Coagulant activity; ANTITUMOR;

D O I：

10.1016/j.carres.2019.107813

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel polysaccharide in Mains halliana Koehne, named MHP-W, was isolated and purified by DEAE-52 cellulose and Sephadex G-100 columns. Structural features were identified by high performance size-exclusion chromatography (HPSEC), fourier transform infrared (FT-IR) spectrometer, gas chromatography (GC) and (1D & 2D) NMR Spectroscopy. Structural characterization showed that the molecular weight of MHP-W was 353 kDa composed of arabinose, xylose, mannose, glucose and galactose in a molar ratio of 2.59: 0.15: 0.23: 0.25: 9.70. The existence of beta-glycosidic bond between the sugar units was confirmed by FT-IR and NMR spectroscopy. The effects of MHP-W on active part thrombin time (APTT), protothrombin time (PT), thrombin time (TT), and fibrinogen (FIB) were screened by a cell-based coagulation activity model. MHP-W could significantly shorten TT (p < 0.001) and increase FIB (p < 0.05) as compared with the control group. The results showed that MHP-W promoted bloodclotting through endogenous and exogenous coagulation pathways as well as increasing fibrinogen content, which indicated that MHP-W had procoagulant activities in vitro.

引用

页数：8

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