Sensitive determination of dissolved sulfide in estuarine water by solid-phase extraction and high-performance liquid chromatography of methylene blue

被引：102

作者：

Tang, DG ^{[1
]}

Santschi, PH ^{[1
]}

机构：

[1] Texas A&M Univ, Dept Oceanog, Galveston, TX 77551 USA

来源：

JOURNAL OF CHROMATOGRAPHY A | 2000年 / 883卷 / 1-2期

关键词：

water analysis; methylene blue; sulfide;

D O I：

10.1016/S0021-9673(00)00381-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A sensitive method involving solid-phase extraction and HPLC analysis of methylene blue has been developed to measure nanomolar levels of dissolved sulfide in oxic surface waters. The procedure included 1) a preconcentration step, in which methylene blue generated from sulfide reaction with n,n-dimethyl-p-phenylenediamine in acidic conditions in the presence of ferric ion was absorbed onto Waters tC(18) cartridge; and 2) a determination step, in which methylene blue was separated by HPLC in a gradient elution to minimize natural organic matter interference and detected by absorbance. The concentrations of the dissolved sulfide, quantified by standard addition, were about 2.1-4.7 nM in oxic surface waters from Galveston Bay, Texas. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：305 / 309

页数：5

共 17 条

[1]

Adams NWH, 1999, ENVIRON TOXICOL CHEM, V18, P2667, DOI [10.1897/1551-5028(1999)018&lt

[2]

2667:SSIWES&gt

[3]

2.3.CO

[4]

2, 10.1002/etc.5620181203]