DETECTABLE BRAF MUTATION IN SERUM DNA SAMPLES FROM PATIENTS WITH PAPILLARY THYROID CARCINOMAS

被引：41

作者：

Chuang, Tony C. Y. ^{[1
]}

Chuang, Alice Y. C. ^{[1
]}

Poeta, Luana ^{[1
]}

Koch, Wayne M. ^{[1
]}

Califano, Joseph A. ^{[1
]}

Tufano, Ralph P. ^{[1
]}

机构：

[1] Johns Hopkins Med Inst, Dept Otolaryngol Head & Neck Surg, Div Head & Neck Canc Res, Baltimore, MD 21287 USA

来源：

HEAD AND NECK-JOURNAL FOR THE SCIENCES AND SPECIALTIES OF THE HEAD AND NECK | 2010年 / 32卷 / 02期

关键词：

BRAF; thyroid carcinoma; serum DNA; biomarker; CANCER-PATIENTS; V600E MUTATION; PLASMA; GENE; ASSOCIATION; DIAGNOSIS; NODULES; MARKER; TUMORS; TOOL;

D O I：

10.1002/hed.21178

中图分类号：

R76 [耳鼻咽喉科学];

学科分类号：

100213 ;

摘要：

Background. An activating point mutation of the BRAF oncogene results in a V600E amino acid missense mutation found in a majority of papillary thyroid carcinomas (PTC). Methods. In this study, 28 matched tumor and serum samples obtained from patients with both benign and malignant thyroid disorders were analyzed for BRAF mutation using a gap-ligase chain reaction technique. Results. The BRAF mutation was absent in tumor DNA samples obtained from patients with benign adenomas, follicular neoplasms or carcinoma, and thyroid lymphoma. In contrast, 5 of 14 PTC tumors were positive for the BRAF mutation. Moreover, 3 of 14 patients with PTC were positive for BRAF mutation in serum and tumor. Of these 3 patients, 2 had lymph node metastasis and 2 had PTC in background of the Hashimoto's thyroiditis. Conclusions. The detection of free circulating mutant BRAF in patients with PTC is possible and future studies are warranted to determine its clinical significance. (C) 2009 Wiley Periodicals, Inc. Head Neck 32: 229-234, 2010

引用

页码：229 / 234

页数：6

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