Site-Specific Cytosine to Uracil Transition by Using Reversible DNA Photo-crosslinking

被引：33

作者：

Fujimoto, Kenzo ^{[1
]}

Konishi-Hiratsuka, Kaoru ^{[1
]}

Sakamoto, Takashi ^{[1
]}

Yoshimura, Yoshinaga ^{[1
]}

机构：

[1] Japan Adv Inst Sci & Technol, Sch Mat Sci, Nomi, Ishikawa 9231292, Japan

来源：

CHEMBIOCHEM | 2010年 / 11卷 / 12期

关键词：

cycloaddition; DNA; gene technology; nucleosides; photochemistry; SINGLE-STRANDED-DNA; CYCLOBUTANE DIMERS; ESCHERICHIA-COLI; THERMAL-RESISTANCE; ACTIVATION-ENERGY; UV-MUTAGENESIS; DEAMINATION; MUTATIONS; PHOTOREACTIVATION; 5-METHYLCYTOSINE;

D O I：

10.1002/cbic.201000274

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

(Chemical Presented) Targeting a single cytosine in the presence of others: The deamination coupled with 3-cyanovinylcarbazole-nucleoside-mediated photo-crosslinking allows heat-induced conversion of cytosine into uracil with high site-specificity and without side reactions. © 2010 Wiley-VCH Verlag GmbH& Co. KGaA.

引用

页码：1661 / 1664

页数：4

共 27 条

[1] Activation-induced cytidine deaminase deaminates deoxycytidine on single-stranded DNA but requires the action of RNase [J].

Bransteitter, R ;

Pham, P ;

Scharff, MD ;

Goodman, MF .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (07) :4102-4107

[2] BISULFITE INDUCES TANDEM DOUBLE CC-]TT MUTATIONS IN DOUBLE-STRANDED DNA .2. KINETICS OF CYTOSINE DEAMINATION [J].

CHEN, H ;

SHAW, BR .

BIOCHEMISTRY, 1994, 33 (14) :4121-4129

[3] Chemistry of glycosylases and endonucleases involved in base-excision repair [J].

David, SS ;

Wiliams, SD .

CHEMICAL REVIEWS, 1998, 98 (03) :1221-1261

[4] Altering the pathway of immunoglobulin hypermutation by inhibiting uracil-DNA glycosylase [J].

Di Noia, J ;

Neuberger, MS .

NATURE, 2002, 419 (6902) :43-48

[5] MUTAGENIC DEAMINATION OF CYTOSINE RESIDUES IN DNA [J].

DUNCAN, BK ;

MILLER, JH .

NATURE, 1980, 287 (5782) :560-561

[6] THERMAL-RESISTANCE TO PHOTOREACTIVATION OF SPECIFIC MUTATIONS POTENTIATED IN ESCHERICHIA-COLI B-R UNG BY ULTRAVIOLET-LIGHT [J].

FIX, D ;

BOCKRATH, R .

MOLECULAR & GENERAL GENETICS, 1981, 182 (01) :7-11

[7] THERMAL-RESISTANCE OF UV-MUTAGENESIS TO PHOTOREACTIVATION IN ESCHERICHIA-COLI-B/R UVRA UNG - ESTIMATE OF ACTIVATION-ENERGY AND FURTHER ANALYSIS [J].

FIX, DF .

MOLECULAR & GENERAL GENETICS, 1986, 204 (03) :452-456

[8] CYTOSINE DEAMINATION IN MISMATCHED BASE-PAIRS [J].

FREDERICO, LA ;

KUNKEL, TA ;

SHAW, BR .

BIOCHEMISTRY, 1993, 32 (26) :6523-6530

[9] A SENSITIVE GENETIC ASSAY FOR THE DETECTION OF CYTOSINE DEAMINATION - DETERMINATION OF RATE CONSTANTS AND THE ACTIVATION-ENERGY [J].

FREDERICO, LA ;

KUNKEL, TA ;

SHAW, BR .

BIOCHEMISTRY, 1990, 29 (10) :2532-2537

[10] A GENOMIC SEQUENCING PROTOCOL THAT YIELDS A POSITIVE DISPLAY OF 5-METHYLCYTOSINE RESIDUES IN INDIVIDUAL DNA STRANDS [J].

FROMMER, M ;

MCDONALD, LE ;

MILLAR, DS ;

COLLIS, CM ;

WATT, F ;

GRIGG, GW ;

MOLLOY, PL ;

PAUL, CL .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (05) :1827-1831

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