Site-Specific Cytosine to Uracil Transition by Using Reversible DNA Photo-crosslinking

被引：31
|
作者：
Fujimoto, Kenzo [1 ]
Konishi-Hiratsuka, Kaoru [1 ]
Sakamoto, Takashi [1 ]
Yoshimura, Yoshinaga [1 ]
机构：
[1] Japan Adv Inst Sci & Technol, Sch Mat Sci, Nomi, Ishikawa 9231292, Japan
来源：
CHEMBIOCHEM | 2010年 / 11卷 / 12期
关键词：
cycloaddition; DNA; gene technology; nucleosides; photochemistry; SINGLE-STRANDED-DNA; CYCLOBUTANE DIMERS; ESCHERICHIA-COLI; THERMAL-RESISTANCE; ACTIVATION-ENERGY; UV-MUTAGENESIS; DEAMINATION; MUTATIONS; PHOTOREACTIVATION; 5-METHYLCYTOSINE;
D O I：
10.1002/cbic.201000274
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
(Chemical Presented) Targeting a single cytosine in the presence of others: The deamination coupled with 3-cyanovinylcarbazole-nucleoside-mediated photo-crosslinking allows heat-induced conversion of cytosine into uracil with high site-specificity and without side reactions. © 2010 Wiley-VCH Verlag GmbH& Co. KGaA.
引用
收藏
页码：1661 / 1664
页数：4
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