Illuminating zinc in biological systems

被引：297
作者：
Lim, NC
Freake, HC
Brückner, C
机构：
[1] Univ Connecticut, Dept Chem, Storrs, CT 06269 USA
[2] Univ Connecticut, Dept Nutr Sci, Storrs, CT 06269 USA
来源：
CHEMISTRY-A EUROPEAN JOURNAL | 2005年 / 11卷 / 01期
关键词：
chemosensors; fluorescent probes; imaging agents; sensors; zinc;
D O I：
10.1002/chem.200400599
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Zinc is the second most abundant transition metal in the human body, fulfilling a multitude of biological roles, but the mechanisms underlying its physiology are poorly understood. The lack of knowledge is, in part, due to the hitherto limited techniques available to track zinc in biological systems. The recent emergence of a number of zinc-specific molecular sensors has provided a new tool to image zinc in live cells and tissue samples. This contribution highlights the concepts behind using zinc-specific fluorescent molecular sensors to gain information about zinc action in biological samples, and provides representative examples of images recorded.
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页码：38 / 49
页数：12
相关论文
共 56 条
[1]   A new zinc(II) fluorophore 2-(9-anthrylmethylamino)ethyl-appended 1,4,7,10-tetraazacyclododecane [J].
Aoki, S ;
Kaido, S ;
Fujioka, H ;
Kimura, E .
INORGANIC CHEMISTRY, 2003, 42 (04) :1023-1030
[2]   Zinc coordination sphere in biochemical zinc sites [J].
Auld, DS .
BIOMETALS, 2001, 14 (3-4) :271-313
[3]  
Belfield KD, 2000, J PHYS ORG CHEM, V13, P837, DOI 10.1002/1099-1395(200012)13:12<837::AID-POC315>3.0.CO
[4]  
2-5
[5]   Fluorescent sensors for Zn2+ based on a fluorescein platform:: Synthesis, properties and intracellular distribution [J].
Burdette, SC ;
Walkup, GK ;
Spingler, B ;
Tsien, RY ;
Lippard, SJ .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2001, 123 (32) :7831-7841
[6]   ZP4, an improved neuronal Zn2+ sensor of the Zinpyr family [J].
Burdette, SC ;
Frederickson, CJ ;
Bu, WM ;
Lippard, SJ .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2003, 125 (07) :1778-1787
[7]   Meeting of the minds: Metalloneurochemistry [J].
Burdette, SC ;
Lippard, SJ .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (07) :3605-3610
[8]   The rhodafluor family.: An initial study of potential ratiometric fluorescent sensors for Zn2+ [J].
Burdette, SC ;
Lippard, SJ .
INORGANIC CHEMISTRY, 2002, 41 (25) :6816-6823
[9]   A tautomeric zinc sensor for ratiometric fluorescence imaging: Application to nitric oxide-induced release of intracellular zinc [J].
Chang, CJ ;
Jaworski, J ;
Nolan, EM ;
Sheng, M ;
Lippard, SJ .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (05) :1129-1134
[10]   A selective, fluorescent sensor for Zn2+ [J].
Chen, Y ;
Zeng, DX .
CHEMPHYSCHEM, 2004, 5 (04) :564-566
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