Unfolding and inactivation of Ampullarium crossean β-glucosidase during denaturation by guanidine hydrochloride

被引：13

作者：

Chen, QX ^{[1
]}

Zhang, Z

Huang, H

Zhao, FK

Xu, GJ

机构：

[1] Xiamen Univ, Sch Life Sci, Dept Biol, Xiamen 361005, Peoples R China

[2] Chinese Acad Sci, Shanghai Inst Biochem, Shanghai 200031, Peoples R China

来源：

INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY | 2003年 / 35卷 / 08期

关键词：

beta-glucosidase; denaturation; inactivation; kinetics; guanidine hydrochloride;

D O I：

10.1016/S1357-2725(02)00266-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Changes of activity and conformation of Ampullarium crossean beta-glucosidase in different concentrations of guanidine hydrochloride (GuHCl) have been studied by measuring the fluorescence spectra and its relative activity after denaturation. The fluorescence intensity of the enzyme decreased distinctly with increasing guanidine concentrations, the emission peaks appeared red shifted (from 338.4 to 350.8 nm), whereas a new fluorescence emission peak appeared near 310 nm. Changes in the conformation and catalytic activity of the enzyme were compared. A corresponding rapid decrease in catalytic activity of the enzyme was also observed. The extent of inactivation was greater than that of conformational changes, indicating that the active site of the enzyme is more flexible than the whole enzyme molecule. k(+0) > k'(+0) also showed that the enzyme was protected by substrate to a certain extent during guanidine denaturation. (C) 2002 Published by Elsevier Science Ltd.

引用

页码：1227 / 1233

页数：7

共 50 条

[41] PH-DEPENDENCE OF THE REVERSIBLE UNFOLDING OF OVALBUMIN A1 BY GUANIDINE-HYDROCHLORIDE [J].

AHMAD, F ;

SALAHUDDIN, A .

BIOCHIMICA ET BIOPHYSICA ACTA, 1979, 576 (02) :333-338

[42] Thermal and guanidine hydrochloride-induced denaturation of human cystatin C [J].

Jankowska, E ;

Wiczk, W ;

Grzonka, Z .

EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS, 2004, 33 (05) :454-461

[43] DISSOCIATION AND AGGREGATION OF D-GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE DURING DENATURATION BY GUANIDINE-HYDROCHLORIDE [J].

LIANG, SJ ;

LIN, YZ ;

ZHOU, JM ;

TSOU, CL ;

WU, PQ ;

ZHOU, ZK .

BIOCHIMICA ET BIOPHYSICA ACTA, 1990, 1038 (02) :240-246

[44] DSC study of cold and heat denaturation processes of β-lactoglobulin A with guanidine hydrochloride [J].

王邦宁 ;

谈夫 .

Science in China(Series B), 1997, (03) :316-322

[45] DSC study of cold and heat denaturation processes of β-lactoglobulin A with guanidine hydrochloride [J].

Bangning Wang ;

Fu Tan .

Science in China Series B: Chemistry, 1997, 40 :316-322

[46] Thermal and guanidine hydrochloride-induced denaturation of human cystatin C [J].

Elzbieta Jankowska ;

Wiesław Wiczk ;

Zbigniew Grzonka .

European Biophysics Journal, 2004, 33 :454-461

[47] Bromophenol blue binding as a probe to study urea and guanidine hydrochloride denaturation of bovine serum albumin [J].

Halim, Adyani Azizah Abd. ;

Kadir, Habsah Abdul ;

Tayyab, Saad .

JOURNAL OF BIOCHEMISTRY, 2008, 144 (01) :33-38

[48] Guanidine hydrochloride and urea-induced unfolding of Brugia malayi hexokinase [J].

Alok Ranjan Singh ;

Shweta Joshi ;

Rahul Arya ;

Arvind Mohan Kayastha ;

Jitendra Kumar Saxena .

European Biophysics Journal, 2010, 39 :289-297

[49] Guanidine hydrochloride and urea-induced unfolding of Brugia malayi hexokinase [J].

Singh, Alok Ranjan ;

Joshi, Shweta ;

Arya, Rahul ;

Kayastha, Arvind Mohan ;

Saxena, Jitendra Kumar .

EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS, 2010, 39 (02) :289-297

[50] SPIN-LABELING PROBE ON CONFORMATIONAL CHANGE AT THE ACTIVE-SITES OF CREATINE-KINASE DURING DENATURATION BY GUANIDINE-HYDROCHLORIDE [J].

LIU, ZJ ;

ZHOU, JM .

BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY, 1995, 1253 (01) :63-68

← 1 2 3 4 5 →