Unfolding and inactivation of Ampullarium crossean β-glucosidase during denaturation by guanidine hydrochloride

被引：13

作者：

Chen, QX ^{[1
]}

Zhang, Z

Huang, H

Zhao, FK

Xu, GJ

机构：

[1] Xiamen Univ, Sch Life Sci, Dept Biol, Xiamen 361005, Peoples R China

[2] Chinese Acad Sci, Shanghai Inst Biochem, Shanghai 200031, Peoples R China

来源：

INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY | 2003年 / 35卷 / 08期

关键词：

beta-glucosidase; denaturation; inactivation; kinetics; guanidine hydrochloride;

D O I：

10.1016/S1357-2725(02)00266-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Changes of activity and conformation of Ampullarium crossean beta-glucosidase in different concentrations of guanidine hydrochloride (GuHCl) have been studied by measuring the fluorescence spectra and its relative activity after denaturation. The fluorescence intensity of the enzyme decreased distinctly with increasing guanidine concentrations, the emission peaks appeared red shifted (from 338.4 to 350.8 nm), whereas a new fluorescence emission peak appeared near 310 nm. Changes in the conformation and catalytic activity of the enzyme were compared. A corresponding rapid decrease in catalytic activity of the enzyme was also observed. The extent of inactivation was greater than that of conformational changes, indicating that the active site of the enzyme is more flexible than the whole enzyme molecule. k(+0) > k'(+0) also showed that the enzyme was protected by substrate to a certain extent during guanidine denaturation. (C) 2002 Published by Elsevier Science Ltd.

引用

页码：1227 / 1233

页数：7

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