Performance of Single and Two-Stage Cross-Flow Ultrafiltration Membrane in Fractionation of Peptide from Microalgae Protein Hydrolysate (Nannochloropsis gaditana)

被引:4
作者
Saleh, Nur Izzati Md [1 ]
Ghani, Wan Azlina Wan Ab Karim [1 ,2 ]
Kamal, Siti Mazlina Mustapa [3 ]
Harun, Razif [1 ]
机构
[1] Univ Putra Malaysia, Fac Engn, Sustainable Proc Engn Res Ctr SPERC, Dept Chem & Environm Engn, Upm Serdang 43400, Malaysia
[2] Univ Putra Malaysia, Inst Plantat Studies, Upm Serdang 43400, Malaysia
[3] Univ Putra Malaysia, Fac Engn, Dept Proc & Food Engn, Upm Serdang 43400, Malaysia
基金
英国工程与自然科学研究理事会;
关键词
microalgae protein hydrolysate; peptide; cross-flow ultrafiltration membrane; permeate flux; peptide transmission; WHEY-PROTEIN; PORE-SIZE; BIOACTIVE PEPTIDES; ELECTRODIALYSIS; FILTRATION; MILK; ADSORPTION; COMPONENTS; RESISTANCE; SEPARATION;
D O I
10.3390/pr9040610
中图分类号
TQ [化学工业];
学科分类号
0817 ;
摘要
Cross-flow ultrafiltration (UF) membrane with two different configurations; single (10 kDa and 5 kDa) and two-stage (10/5 kDa) in fractionating microalgae protein hydrolysate (MPH) were studied to obtain a low molecular weight of peptide. The effect of flow rate, trans-membrane pressure (TMP), and pH in fractionating MPH were evaluated based on permeate flux and peptide transmission. The results showed that, for single UF membrane, optimum operating parameters were at a flow rate of 23 mL/min, TMP of 1.5 bar, and pH of 9, with permeate flux of 43.65 L/m(2) h (10 kDa) and 55.42 L/m(2) h (5 kDa) and peptide transmission of 58.20% (10 kDa) and 67.34% (5 kDa). Meanwhile, for two-stage (10/5 kDa) UF membrane, the best parameters were observed at a flow rate of 23 mL/min, TMP of 1.5 bar, and pH of 2, with permeate flux of 69.85 L/m(2) h and peptide transmission of 79.13%. Fractionation of MPH with two-stage UF membrane was observed to be better at producing a low molecular weight of peptide compared to single UF membrane. In conclusion, it was possible to produce permeate flux with a high amount of low molecular weight of peptide by controlling the operating parameters with the suitable configuration membrane.
引用
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页数:20
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