Performance of Single and Two-Stage Cross-Flow Ultrafiltration Membrane in Fractionation of Peptide from Microalgae Protein Hydrolysate (Nannochloropsis gaditana)

被引：5

作者：

Saleh, Nur Izzati Md ^{[1
]}

Ghani, Wan Azlina Wan Ab Karim ^{[1
,2
]}

Kamal, Siti Mazlina Mustapa ^{[3
]}

Harun, Razif ^{[1
]}

机构：

[1] Univ Putra Malaysia, Fac Engn, Sustainable Proc Engn Res Ctr SPERC, Dept Chem & Environm Engn, Upm Serdang 43400, Malaysia

[2] Univ Putra Malaysia, Inst Plantat Studies, Upm Serdang 43400, Malaysia

[3] Univ Putra Malaysia, Fac Engn, Dept Proc & Food Engn, Upm Serdang 43400, Malaysia

来源：

PROCESSES | 2021年 / 9卷 / 04期

基金：

英国工程与自然科学研究理事会;

关键词：

microalgae protein hydrolysate; peptide; cross-flow ultrafiltration membrane; permeate flux; peptide transmission; WHEY-PROTEIN; PORE-SIZE; BIOACTIVE PEPTIDES; ELECTRODIALYSIS; FILTRATION; MILK; ADSORPTION; COMPONENTS; RESISTANCE; SEPARATION;

D O I：

10.3390/pr9040610

中图分类号：

TQ [化学工业];

学科分类号：

0817 ;

摘要：

Cross-flow ultrafiltration (UF) membrane with two different configurations; single (10 kDa and 5 kDa) and two-stage (10/5 kDa) in fractionating microalgae protein hydrolysate (MPH) were studied to obtain a low molecular weight of peptide. The effect of flow rate, trans-membrane pressure (TMP), and pH in fractionating MPH were evaluated based on permeate flux and peptide transmission. The results showed that, for single UF membrane, optimum operating parameters were at a flow rate of 23 mL/min, TMP of 1.5 bar, and pH of 9, with permeate flux of 43.65 L/m(2) h (10 kDa) and 55.42 L/m(2) h (5 kDa) and peptide transmission of 58.20% (10 kDa) and 67.34% (5 kDa). Meanwhile, for two-stage (10/5 kDa) UF membrane, the best parameters were observed at a flow rate of 23 mL/min, TMP of 1.5 bar, and pH of 2, with permeate flux of 69.85 L/m(2) h and peptide transmission of 79.13%. Fractionation of MPH with two-stage UF membrane was observed to be better at producing a low molecular weight of peptide compared to single UF membrane. In conclusion, it was possible to produce permeate flux with a high amount of low molecular weight of peptide by controlling the operating parameters with the suitable configuration membrane.

引用

页数：20

共 62 条

[1] Isolation and and characterisation of enzymatic hydrolysed peptides with antioxidant activities from green tender sorghum [J].