Suitability of ultra-high performance liquid chromatography for the determination of fat-soluble nutritional status (vitamins A, E, D, and individual carotenoids)

被引：51
作者：
Granado-Lorencio, F. [1 ]
Herrero-Barbudo, C. [1 ]
Blanco-Navarro, I. [1 ]
Perez-Sacristan, B. [1 ]
机构：
[1] Hosp Univ Puerta Hierro Majadahonda, Unidad Vitaminas, Bioquim Clin, Madrid 28222, Spain
来源：
ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2010年 / 397卷 / 03期
关键词：
UHPLC; Retinol; Tocopherol; 25-OH-vitamin D; Carotenoids; Nutritional status; TOCOPHEROL; RETINOL; SERUM;
D O I：
10.1007/s00216-010-3655-2
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Our aim was to assess the suitability of ultra-high performance liquid chromatography (UHPLC) for the simultaneous determination of biomarkers of vitamins A (retinol, retinyl esters), E (alpha- and gamma-tocopherol), D (25-OH-vitamin D), and the major carotenoids in human serum to be used in clinical practice. UHPLC analysis was performed on HSS T3 column (2.1 x 100 mm; 1.8 mu m) using gradient elution and UV-VIS detection. The system allows the simultaneous determination of retinol, retinyl palmitate, 25-OH-vitamin D, alpha- and gamma-tocopherol, lutein plus zeaxanthin, alpha-carotene, beta-carotene, alpha- and beta-cryptoxanthin and lycopene. The method showed a good linearity over the physiological range with an adequate accuracy in samples from quality control programs. Suitability of the method in clinical practice was tested by analyzing samples (n = 286) from patients. In conclusion, UHPLC constitutes a reliable approach for nutrient/biomarker profiling allowing the rapid, simultaneous and low-cost determination of vitamins A, E, and D (including vitamers and ester forms) and the major carotenoids in clinical practice.
引用
收藏
页码：1389 / 1393
页数：5
相关论文
共 15 条
[1]   Passclaim*Consensus on Criteria [J].
Peter J. Aggett ;
Jean-Michel Antoine ;
Nils-Georg Asp ;
France Bellisle ;
Laura Contor ;
John H. Cummings ;
John Howlett ;
Detlef J. G. Müller ;
Christoph Persin ;
Loek T. J. Pijls ;
Gerhard Rechkemmer ;
Sandra Tuijtelaars ;
Hans Verhagen .
European Journal of Nutrition, 2005, 44 (Suppl 1) :i5-i30
[2]   Metabolic profiling of major vitamin D metabolites using Diels-Alder derivatization and ultra-performance liquid chromatography-tandem mass spectrometry [J].
Aronov, Pavel A. ;
Hall, Laura M. ;
Dettmer, Katja ;
Stephensen, Charles B. ;
Hammock, Bruce D. .
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2008, 391 (05) :1917-1930
[3]   Assay variation confounds the diagnosis of hypovitaminosis D: A call for standardization [J].
Binkley, N ;
Krueger, D ;
Cowgill, CS ;
Plum, L ;
Lake, E ;
Hansen, KE ;
DeLuca, HF ;
Drezner, MK .
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 2004, 89 (07) :3152-3157
[4]   Comparison of a novel ultra-performance liquid chromatographic method for determination of retinol and α-tocopherol in human serum with conventional HPLC using monolithic and particulate columns [J].
Citova, I. ;
Havlikova, L. ;
Urbanek, L. ;
Solichova, D. ;
Novakova, L. ;
Solich, P. .
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2007, 388 (03) :675-681
[5]   Testing nutrient profile models in relation to energy density and energy cost [J].
Drewnowski, A. ;
Maillot, M. ;
Darmon, N. .
EUROPEAN JOURNAL OF CLINICAL NUTRITION, 2009, 63 (05) :674-683
[6]  
FIDANZA F, 1994, BIBL NUTR DIET, P9
[7]   Metabolic assessment - a key to nutritional strategies for health [J].
German, JB ;
Watkins, SM .
TRENDS IN FOOD SCIENCE & TECHNOLOGY, 2004, 15 (11) :541-549
[8]   Simultaneous determination of vitamins A, E and 25-OH-vitamin D:: Application in clinical assessments [J].
Granado-Lorencio, F ;
Olmedilla-Alonso, B ;
Herrero-Barbudo, C ;
Blanco-Navarro, I ;
Blázquez-García, S ;
Pérez-Sacristán, B .
CLINICAL BIOCHEMISTRY, 2006, 39 (02) :180-182
[9]   25-OH-Vitamin D assay variation and subject management in clinical practice [J].
Granado-Lorencio, F. ;
Salazar Mosteiro, J. ;
Herrero-Barbudo, C. ;
Donoso Navarro, E. ;
Blanco-Navarro, I. ;
Perez-Sacristan, B. .
CLINICAL BIOCHEMISTRY, 2010, 43 (4-5) :531-533
[10]   A simple automated solid-phase extraction procedure for measurement of 25-hydroxyvitamin D3 and D2 by liquid chromatography-tandem mass spectrometry [J].
Knox, Susan ;
Harris, John ;
Calton, Lisa ;
Wallace, A. Michael .
ANNALS OF CLINICAL BIOCHEMISTRY, 2009, 46 :226-230
12