A protocol for islet isolation from mouse pancreas

被引：272

作者：

Li, Dong-Sheng ^{[1
]}

Yuan, Ya-Hong ^{[1
]}

Tu, Han-Jun ^{[1
]}

Liang, Qing-Le ^{[1
]}

Dai, Long-Jun ^{[1
,2
]}

机构：

[1] Yunyang Med Coll, Hubei Key Lab Embryon Stem Cell Res, Tai He Hosp, Shiyan, Hubei, Peoples R China

[2] Univ British Columbia, Barber Human Islet Transplant Lab, Dept Surg, Vancouver, BC V5Z 1M9, Canada

来源：

NATURE PROTOCOLS | 2009年 / 4卷 / 11期

关键词：

TYPE-1; DIABETES-MELLITUS; TRANSPLANTATION; PURIFICATION; LANGERHANS;

D O I：

10.1038/nprot.2009.150

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Mouse islets are commonly used in diabetes-related studies. Adequate amounts of good quality islets are prerequisites for a reliable investigation. We describe a protocol for islet isolation from mouse pancreas. Three major manipulations are employed in the islet isolation procedure: in situ pancreas perfusion with collagenase, pancreas digestion and islet purification. The whole procedure takes 30-45 min for each individual mouse. By using this protocol, a reasonable number of islets can be obtained in a relatively short period of time. This protocol has been proven to be practicable and reproducible. It can be easily followed by individuals who do not have previous experience in the related research field.

引用

页码：1649 / 1652

页数：4

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