Novosphingobium colocasiae sp nov., isolated from a taro field

A novel bacterial strain, designated Teta-03(T), was isolated from a taro field in Taiwan and characterized using a polyphasic taxonomic approach. Cells of strain Teta-03(T) were aerobic, Gram-stain-negative, rod-shaped and non-motile and formed bright yellow colonies. Growth occurred at 10-37 degrees C (optimum, 20 degrees C), with 0-1.0 % (w/v) NaCl (optimum, 0 %) and at pH 3.0-9.0 (optimum, pH 7.0-8.0). The major fatty acids (>10 %) of strain Teta-03(T) were C-18 : 1 omega 7c, summed feature 3 (C-16 : 1 omega 7c and/or C-16 : 1 omega 6c) and C-16 : 0. The polar lipid profile consisted of phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidyldimethylethanolamine, sphingoglycolipid, phosphatidylcholine, an uncharacterized glycolipid and an uncharacterized aminolipid. The major polyamine was spermidine. The major isoprenoid quinone was Q-10. The DNA G + C content was 65.0 mol%. On the basis of 16S rRNA gene sequence analysis, strain Teta-03(T) was shown to belong to the genus Novosphingobium and showed highest similarity to Novosphingobium barchaimii LL02(T) (96.8 %). Phenotypic characteristics of the novel strain also differed from those of the closest related species of the genus Novosphingobium. On the basis of the genotypic, chemotaxonomic and phenotypic data, strain Teta-03(T) represents a novel species of the genus Novosphingobium, for which the name Novosphingobium colocasiae sp. nov. is proposed. The type strain is Teta-03(T) (=LMG 27385(T) =KCTC 32255(T)).