Continuous flow generation of magnetoliposomes in a low-cost portable microfluidic platform

被引：12

作者：

Conde, Alvaro J. ^{[1
]}

Batalla, Milena ^{[2
]}

Cerda, Belen ^{[2
]}

Mykhaylyk, Olga ^{[3
]}

Plank, Christian ^{[3
]}

Podhajcer, Osvaldo ^{[4
]}

Cabaleiro, Juan M. ^{[5
,6
]}

Madrid, Rossana E. ^{[1
]}

Policastro, Lucia ^{[2
]}

机构：

[1] Univ Nacl Tucuman, CONICET, Inst Super Invest Biol, LAMEIN,Dept Bioingn,Fac Ciencias Exacts & Technol, RA-4000 San Miguel De Tucuman, Tucuman, Argentina

[2] Comis Nacl Energia Atom, CONICET, Lab Nanomed, Inst Nanociencia & Nanotecnol, RA-1429 Buenos Aires, DF, Argentina

[3] Tech Univ Munich, Inst Expt Oncol & Therapy Res, D-81675 Munich, Germany

[4] Fdn Inst Leloir, Lab Terapia Mol & Celular, Buenos Aires, DF, Argentina

[5] Univ Buenos Aires, Fac Ingn, CONICET, Lab Fluidodinam, Buenos Aires, DF, Argentina

[6] Univ Marina Mercante, Lab Micro & Nanofluid & Plasma, Buenos Aires, DF, Argentina

来源：

LAB ON A CHIP | 2014年 / 14卷 / 23期

关键词：

GENE DELIVERY; IN-VITRO; LIPOSOMES; CHEMOTHERAPY; NANOPARTICLES; VESICLES; DEVICES; DESIGN; SYSTEM;

D O I：

10.1039/c4lc00839a

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We present a low-cost, portable microfluidic platform that uses laminated polymethylmethacrylate chips, peristaltic micropumps and LEGO (R) Mindstorms components for the generation of magnetoliposomes that does not require extrusion steps. Mixtures of lipids reconstituted in ethanol and an aqueous phase were injected independently in order to generate a combination of laminar flows in such a way that we could effectively achieve four hydrodynamic focused nanovesicle generation streams. Monodisperse magnetoliposomes with characteristics comparable to those obtained by traditional methods have been obtained. The magnetoliposomes are responsive to external magnetic field gradients, a result that suggests that the nanovesicles can be used in research and applications in nanomedicine.

引用

页码：4506 / 4512

页数：7

共 31 条

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