Low noise, self-phase-modulation-enabled femtosecond fiber sources tunable in 740-1236 nm for wide two-photon fluorescence microscopy applications

被引：13

作者：

Chou, Lu-Ting ^{[1
,2
]}

Liu, Yu-Cheng ^{[1
]}

Zhong, Dong-Lin ^{[1
]}

Lin, Wei-Zhong ^{[1
,2
]}

Hung, Hao-Hsuan ^{[1
,2
]}

Chan, Chao-Jin ^{[1
,2
]}

Chen, Zi-Ping ^{[1
,2
]}

Chia, Shih-Hsuan ^{[1
,2
]}

机构：

[1] Natl Yang Ming Univ, Inst Biophoton, 155,Linong St,Linong St, Taipei 11221, Taiwan

[2] Natl Yang Ming Chiao Tung Univ, Inst Biophoton, 155,Sec 2,Linong St, Taipei 11221, Taiwan

来源：

BIOMEDICAL OPTICS EXPRESS | 2021年 / 12卷 / 05期

关键词：

MEGAWATT PEAK POWER; LASER SOURCES; LIVING CELLS; EXCITATION; PULSES; TISSUE; SENSITIVITY; GENERATION; DISPERSION; DYNAMICS;

D O I：

10.1364/BOE.422668

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We have demonstrated widely tunable Yb:fiber-based laser sources, aiming to replace Ti:sapphire lasers for the nJ-level ultrafast applications, especially for the uses of nonlinear light microscopy. We investigated the influence of different input parameters to obtain an expansive spectral broadening, enabled by self-phase modulation and further reshaped by self-steepening, in the normal dispersion regime before the fiber damage. We also discussed the compressibility and intensity fluctuations of the demonstrated pulses, to reach the transform-limited duration with a very low intensity noise. Most importantly, we have demonstrated clear two-photon fluorescence images from UV-absorbing fluorophores to deep red dye stains. (c) 2021 Optical Society of America under the terms of the OSA Open Access Publishing Agreement

引用

页码：2888 / 2901

页数：14

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