The transcriptome of Populus in elevated CO2 reveals increased anthocyanin biosynthesis during delayed autumnal senescence

被引:66
作者
Tallis, M. J. [1 ]
Lin, Y. [1 ]
Rogers, A. [2 ,3 ]
Zhang, J. [1 ,4 ]
Street, N. R. [1 ]
Miglietta, F. [5 ]
Karnosky, D. F. [6 ]
De Angelis, P. [7 ]
Calfapietra, C. [7 ]
Taylor, G. [1 ]
机构
[1] Univ Southampton, Sch Biol Sci, Southampton SO16 7PX, Hants, England
[2] Brookhaven Natl Lab, Dept Environm Sci, Upton, NY 11973 USA
[3] Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA
[4] Peking Univ, Coll Life Sci, Beijing 100871, Peoples R China
[5] CNR, Inst Biometeorol, I-50145 Florence, Italy
[6] Michigan Technol Univ, Sch Forest Resources & Environm Sci, Houghton, MI 49931 USA
[7] Univ Tuscia, Dept Forest Environm & Resources DISAFRI, I-01100 Viterbo, Italy
关键词
anthocyanin biosynthesis; autumnal senescence; cDNA microarray; elevated CO2; LDOX; Populus; SUCROSE-SPECIFIC INDUCTION; DIOXIDE ENRICHMENT FACE; GENE-EXPRESSION; LEAF SENESCENCE; POPLAR PLANTATION; GROWTH CESSATION; DOWN-REGULATION; CARBON; LEAVES; ARABIDOPSIS;
D O I
10.1111/j.1469-8137.2010.03184.x
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The delay in autumnal senescence that has occurred in recent decades has been linked to rising temperatures. Here, we suggest that increasing atmospheric CO2 may partly account for delayed autumnal senescence and for the first time, through transcriptome analysis, identify gene expression changes associated with this delay. Using a plantation of Populus x euramericana grown in elevated [CO2] (e[CO2]) with free-air CO2 enrichment (FACE) technology, we investigated the molecular and biochemical basis of this response. A Populus cDNA microarray was used to identify genes representing multiple biochemical pathways influenced by e[CO2] during senescence. Gene expression changes were confirmed through real-time quantitative PCR, and leaf biochemical assays. Pathways for secondary metabolism and glycolysis were significantly up-regulated by e[CO2] during senescence, in particular, those related to anthocyanin biosynthesis. Expressed sequence tags (ESTs) representing the two most significantly up-regulated transcripts in e[CO2], LDOX (leucoanthocyanidin dioxgenase) and DFR (dihydroflavonol reductase), gave (e[CO2]/ambient CO2 (a[CO2])) expression ratios of 39.6 and 19.3, respectively. We showed that in e[CO2] there was increased autumnal leaf sugar accumulation and up-regulation of genes determining anthocyanin biosynthesis which, we propose, prolongs leaf longevity during natural autumnal senescence.
引用
收藏
页码:415 / 428
页数:14
相关论文
共 73 条
[1]   Testing the "source-sink" hypothesis of down-regulation of photosynthesis in elevated [CO2] in the field with single gene substitutions in Glycine max [J].
Ainsworth, EA ;
Rogers, A ;
Nelson, R ;
Long, SP .
AGRICULTURAL AND FOREST METEOROLOGY, 2004, 122 (1-2) :85-94
[2]   The effects of elevated CO2 concentration on soybean gene expression.: An analysis of growing and mature leaves [J].
Ainsworth, Elizabeth A. ;
Rogers, Alistair ;
Vodkin, Lila O. ;
Walter, Achim ;
Schurr, Ulrich .
PLANT PHYSIOLOGY, 2006, 142 (01) :135-147
[3]   A transcriptional timetable of autumn senescence -: art. no. R24 [J].
Andersson, A ;
Keskitalo, J ;
Sjödin, A ;
Bhalerao, R ;
Sterky, F ;
Wissel, K ;
Tandre, K ;
Aspeborg, H ;
Moyle, R ;
Ohmiya, Y ;
Bhalerao, R ;
Brunner, A ;
Gustafsson, P ;
Karlsson, J ;
Lundeberg, J ;
Nilsson, O ;
Sandberg, G ;
Strauss, S ;
Sundberg, B ;
Uhlen, M ;
Jansson, S ;
Nilsson, P .
GENOME BIOLOGY, 2004, 5 (04)
[4]   CONTROLLING THE FALSE DISCOVERY RATE - A PRACTICAL AND POWERFUL APPROACH TO MULTIPLE TESTING [J].
BENJAMINI, Y ;
HOCHBERG, Y .
JOURNAL OF THE ROYAL STATISTICAL SOCIETY SERIES B-STATISTICAL METHODOLOGY, 1995, 57 (01) :289-300
[5]   CO/FT regulatory module controls timing of flowering and seasonal growth cessation in trees [J].
Böhlenius, H ;
Huang, T ;
Charbonnel-Campaa, L ;
Brunner, AM ;
Jansson, S ;
Strauss, SH ;
Nilsson, O .
SCIENCE, 2006, 312 (5776) :1040-1043
[6]   Gene expression and metabolite profiling of Populus euphratica growing in the Negev desert -: art. no. R101 [J].
Brosché, M ;
Vinocur, B ;
Alatalo, ER ;
Lamminmäki, A ;
Teichmann, T ;
Ottow, EA ;
Djilianov, D ;
Afif, D ;
Bogeat-Triboulot, MB ;
Altman, A ;
Polle, A ;
Dreyer, E ;
Rudd, S ;
Lars, P ;
Auvinen, P ;
Kangasjärvi, J .
GENOME BIOLOGY, 2005, 6 (12)
[7]   Photosynthetic acclimation to elevated CO2 is modified by source:sink balance in three component species of chalk grassland swards grown in a free air carbon dioxide enrichment (FACE) experiment [J].
Bryant, J ;
Taylor, G ;
Frehner, M .
PLANT CELL AND ENVIRONMENT, 1998, 21 (02) :159-168
[8]   Comparative transcriptome analysis reveals significant differences in gene expression and signalling pathways between developmental and dark/starvation-induced senescence in Arabidopsis [J].
Buchanan-Wollaston, V ;
Page, T ;
Harrison, E ;
Breeze, E ;
Lim, PO ;
Nam, HG ;
Lin, JF ;
Wu, SH ;
Swidzinski, J ;
Ishizaki, K ;
Leaver, CJ .
PLANT JOURNAL, 2005, 42 (04) :567-585
[9]   The molecular analysis of leaf senescence - a genomics approach [J].
Buchanan-Wollaston, V ;
Earl, S ;
Harrison, E ;
Mathas, E ;
Navabpour, S ;
Page, T ;
Pink, D .
PLANT BIOTECHNOLOGY JOURNAL, 2003, 1 (01) :3-22
[10]  
Chang S. J., 1993, Plant Molecular Biology Reporter, V11, P113, DOI 10.1007/BF02670468