Higher HLA class I expression in renal cell carcinoma than in autologous normal tissue

被引：19

作者：

Saenz-Lopez, P. ^{[1
]}

Gouttefangeas, C. ^{[2
]}

Hennenlotter, J. ^{[3
]}

Concha, A. ^{[4
]}

Maleno, I. ^{[1
]}

Ruiz-Cabello, F. ^{[1
,5
]}

Cozar, J. M. ^{[6
]}

Tallada, M. ^{[6
]}

Stenzl, A. ^{[3
]}

Rammensee, H. -G. ^{[2
]}

Garrido, F. ^{[1
,5
]}

Cabrera, T. ^{[1
,5
]}

机构：

[1] Hosp Univ Virgen Nieves, Serv Anal Clin, Granada 18014, Spain

[2] Univ Tubingen, Inst Cell Biol, Dept Immunol, Tubingen, Germany

[3] Univ Tubingen Hosp, Urol Clin, Tubingen, Germany

[4] Hosp Univ Virgen Nieves, Serv Anat Patol, Granada 18014, Spain

[5] Univ Granada, Dept Bioquim & Biol Mol Inmunol 3, Granada, Spain

[6] Hosp Univ Virgen Nieves, Serv Urol, Granada 18014, Spain

来源：

TISSUE ANTIGENS | 2010年 / 75卷 / 02期

关键词：

human leukocyte antigen class I; immunohistochemistry; microdissection; normal renal tissue; renal cell carcinoma; CHROMOSOME REGION 6P21; HIGH-FREQUENCY; DOWN-REGULATION; MELANOMA-CELLS; COLORECTAL CARCINOMAS; LARYNGEAL CARCINOMAS; ALTERED PHENOTYPES; CERVICAL-CANCER; ANTIGEN; TUMORS;

D O I：

10.1111/j.1399-0039.2009.01409.x

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

A total of 93 frozen primary renal cell carcinoma (RCC) samples and 31 frozen samples of corresponding normal renal tissue were analyzed for human leukocyte antigen (HLA) class I and HLA-DR expression. Unexpectedly, HLA class I expression was much higher on RCC cells than on normal renal tubular cells. Immunohistochemistry analysis of frozen and paraffin-embedded tissue samples, applying an extended panel of specific anti-HLA monoclonal antibodies, showed elevated HLA class I antigen expression in 95.6% of the tumors vs only 12.9% of normal renal tissues. These findings were confirmed by molecular analysis of HLA heavy chain and beta 2-microglobulin (beta 2m) transcription levels using quantitative real-time polymerase chain reaction (PCR) on microdissected tissue samples (isolated tumor nests and autologous normal renal tubules) from four patients. These results might help to explain the relatively high success rate of immunotherapy in patients with RCC. The molecular mechanism underlying the increased HLA class I expression in RCC has yet to be elucidated.

引用

页码：110 / 118

页数：9

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