Occurrence and species distribution of pathogenic Mucorales in unselected soil samples from France

被引：22

作者：

Mousavi, B. ^{[1
,2
]}

Costa, J. M. ^{[3
]}

Arne, P. ^{[1
]}

Guillot, J. ^{[1
]}

Chermette, R. ^{[1
]}

Botterel, F. ^{[1
]}

Dannaoui, E. ^{[1
,4
]}

机构：

[1] Paris Est Creteil Univ, Dynamyc Res Grp, Ecole Natl Vet Alfort, EA 7380, Creteil, France

[2] Mazandaran Univ Med Sci, Sch Med, Invas Fungi Res Ctr, Dept Mol & Med Mycol, Sari, Iran

[3] Lab CERBA, St Ouen, France

[4] Paris Descartes Univ, European Georges Pompidou Hosp, AP HP, Fac Med,Parasitol Mycol Unit,Microbiol Dept, Paris, France

来源：

MEDICAL MYCOLOGY | 2018年 / 56卷 / 03期

关键词：

Mucorales; soil; molecular identification; antifungal susceptibility testing; IN-VITRO SUSCEPTIBILITIES; ANTIFUNGAL SUSCEPTIBILITY; CUTANEOUS MUCORMYCOSIS; AMPHOTERICIN-B; POSACONAZOLE; ASPERGILLUS; INFECTIONS; DIAGNOSIS; EUCAST; DNA;

D O I：

10.1093/mmy/myx051

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Mucormycosis is a life-threatening invasive fungal disease that affects a variety of patient groups. Although Mucorales are mostly opportunistic pathogens originating from soil or decaying vegetation, there are currently few data on prevalence of this group of fungi in the environment. The aim of the present study was to assess the prevalence and diversity of species of Mucorales from soil samples collected in France. Two grams of soil were homogenized in sterile saline and plated on Sabouraud dextrose agar and RPMI agar supplemented with itraconazole or voriconazole. Both media contained chloramphenicol and gentamicin. The plates were incubated at 35 +/- 2 degrees C and checked daily for fungal growth for a maximum of 7 d. Mucorales were subcultured for purity. Each isolate was identified phenotypically and molecular identification was performed by ITS sequencing. A total of 170 soil samples were analyzed. Forty-one isolates of Mucorales were retrieved from 38 culture-positive samples. Among the recovered isolates, 27 Rhizopus arrhizus,11 Mucor circinelloides, one Lichtheimia corymbifera, one Rhizopus microsporus and one Cunninghamella bertholletiae were found. Positive soil samples came from cultivated fields but also from other types of soil such as flower beds. Mucorales were retrieved from samples obtained in different geographical regions of France. Voriconazole-containing medium improved the recovery of Mucorales compared with other media. The present study showed that pathogenic Mucorales are frequently recovered from soil samples in France. Species diversity should be further analyzed on a larger number of soil samples from different geographic areas in France and in other countries.

引用

页码：315 / 321

页数：7

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