Antiviral Activity of Daphnoretin Isolated from Wikstroemia indica

被引：53

作者：

Ho, Wing-Shan ^{[2
]}

Xue, Jun-Yi ^{[2
]}

Sun, Samuel S. M. ^{[2
]}

Ooi, Vincent E. C. ^{[2
]}

Li, Yao-Lan ^{[1
]}

机构：

[1] Jinan Univ, Inst Tradit Chinese Med & Nat Prod, Coll Pharm, Guangzhou, Guangdong, Peoples R China

[2] Chinese Univ Hong Kong, Dept Biol, Shatin, Hong Kong, Peoples R China

来源：

PHYTOTHERAPY RESEARCH | 2010年 / 24卷 / 05期

关键词：

antiviral; daphnoretin; Wikstroemia indica; RSV; PROTEIN-KINASE-C; AGENTS; MECHANISMS; ACTIVATOR; ROOTS;

D O I：

10.1002/ptr.2935

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

The ethanol extract of Wikstroemia indica was fractionated with organic solvents of different polarities, and various fractions were screened for their antiviral activity against respiratory syncytial virus (RSV) using a cytopathic effect (CPE) reduction assay. The ethyl acetate fraction was most active against RSV with 50% inhibition concentration (IC50) value < 3.9 mu g/mL and a selectivity index (SI) > 64.1. Further isolation and purification of the fraction led to a purified compound, daphnoretin. Daphnoretin was tested for its anti-RSV activity using a plaque reduction assay and found active against RSV, with an IC50 value of 5.87 mu g/mL and SI value of 28.17. The mode of antiviral action study revealed that daphnoretin could slightly inhibit the early events of the viral infection but its effect was mainly on the later phase of the replication cycle. Copyright (C) 2009 John Wiley & Sons, Ltd.

引用

页码：657 / 661

页数：5

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