Production and characterization of monoclonal antibodies to Newcastle Disease Virus

被引：0

作者：

Kumar, G. Ravi ^{[1
]}

Saxena, Shikha ^{[1
]}

Sahoo, A. P. ^{[1
]}

Chaturvedi, Uttara ^{[1
]}

Kumar, Satish ^{[1
]}

Santra, Lakshman ^{[1
]}

Desai, G. S. ^{[1
]}

Singh, Lakshyaveer ^{[1
]}

Tiwari, Ashok K. ^{[1
]}

机构：

[1] Indian Vet Res Inst, Dept Vet Biotechnol, Mol Biol Lab, Izatnagar 243122, Uttar Pradesh, India

来源：

INDIAN JOURNAL OF EXPERIMENTAL BIOLOGY | 2016年 / 54卷 / 03期

关键词：

H N gene; IFAT; NDV; Poultry; RNA virus;

D O I：

暂无

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Newcastle Disease (ND) is one of the major causes of economic loss in the poultry industry. Newcastle Disease Virus (NDV) is a single-stranded, negative-sense enveloped RNA virus (Fam. Paramyxoviridae; Order Mononegavirales). In the present study three monoclonal antibodies (MAbs) were produced by polyethyleneglycol (PEG)-mediated fusion of lymphocytes sensitized to NDV Bareilly strain and myeloma cells. NDV possesses ability to agglutinate erythrocytes of avian species. All the three MAbs designated as 2H7, 3E9 and 3G6 caused hemagglutination inhibition of NDV by specifically binding to NDV. The reactivity for all the 3 MAbs on indirect ELISA was found to be significantly higher than the antibody and antigen controls. On flowcytometry of HeLa cells infected with NDV using the MAbs as primary antibodies, there was a significant difference in the percentage of cells showing positive fluorescence compared to the mock control. One of the MAbs (3E9) was found to react with hemagglutinin-neuraminidase (RN) protein on western blot.

引用

页码：180 / 186

页数：7

共 30 条

[1] Alexander DJ, 1991, NEWCASTLE DIS OTHER, P519
[2] Alexander DJ, 1989, NEWCASTLE DIS LAB MA, P120
[3] Alexander DJ, 1988, NEWCASTLE DIS, P10
[4] Flow cytometric detection of viruses
Brussaard, CPD
Marie, D
Bratbak, G
[J]. JOURNAL OF VIROLOGICAL METHODS, 2000, 85 (1-2) : 175 - 182
[5] Chaturvedi U, 2013, INDIAN J BIOTECHNOL, V12, P425
[6] DEDUCED AMINO-ACID-SEQUENCES AT THE FUSION PROTEIN CLEAVAGE SITE OF NEWCASTLE-DISEASE VIRUSES SHOWING VARIATION IN ANTIGENICITY AND PATHOGENICITY
COLLINS, MS
BASHIRUDDIN, JB
ALEXANDER, DJ
[J]. ARCHIVES OF VIROLOGY, 1993, 128 (3-4) : 363 - 370
[7] Detection and differentiation of pathogenicity of avian paramyxovirus serotype 1 from field cases using one-step reverse transcriptase-polymerase chain reaction
Creelan, JL
Graham, DA
McCullough, SJ
[J]. AVIAN PATHOLOGY, 2002, 31 (05) : 493 - 499
[8] Complete nucleotide sequence of Newcastle disease virus:: evidence for the existence of a new genus within the subfamily Paramyxovirinae
de Leeuw, O
Peeters, B
[J]. JOURNAL OF GENERAL VIROLOGY, 1999, 80 : 131 - 136
[9] Fenner F, 1987, VET VIROLOGY, P588
[10] A dot immunoblotting assay (dot blot ELISA) for early detection of Newcastle disease antibodies in chickens
Folitse, R
Halvorson, DA
Sivanandan, V
[J]. AVIAN DISEASES, 1998, 42 (01) : 14 - 19

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