Physicochemical properties of aqueous mixed solutions of sugar-persubstituted poly(amidoamine)dendrimers and bovine serum albumin

The interactions of bovine serum albumin (BSA) with sugar-persubstituted poly(amidoamine)dendrimers (SB, generation 3 and 5) have been studied using several techniques including interfacial rheometer. The sample solutions are prepared by mixing SB5 or SB3 to BSA under a fixed concentration of BSA. In BSA-SB5 system, mixed solutions become turbid due to the formation of aggregates between BSA and SB5 by adding a small amount of SB5, but a further addition of SB5 renders the aggregates redispersed. These results are reflected in the changes of surface tension, interfacial rheology, and circular dichroism (CD) spectra for BSA-SB5 system. On the other hand, BSA-SB3 system does not show significant physicochemical properties due to a weak interaction between BSA and SB3. (C) 2002 Elsevier Science B.V. All rights reserved.