Development and validation of a liquid chromatography-tandem mass spectrometric method for the determination of α-methyltyrosine in human plasma

被引:4
作者
Humphries, D [1 ]
Ruterbories, K
Chan, C
Narayanan, R
机构
[1] Eli Lilly & Co, Lilly Corp Ctr, Dept Drug Disposit, Indianapolis, IN 46285 USA
[2] Natl Univ Singapore, Lilly NUS Ctr Clin Pharmacol Pte Ltd, Singapore 117597, Singapore
来源
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2004年 / 810卷 / 02期
关键词
alpha-methyltyrosine; validation;
D O I
10.1016/j.jchromb.2004.08.004
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A sensitive and selective LC-MS-MS method for the isolation and quantification of alpha-methyltyrosine (AMT) from human plasma is described. The method employs a simple protein precipitation using zinc sulfate and sodium hydroxide. This precipitation procedure produced samples with high aqueous content that could be directly injected into a LC-MS-MS system without compromising reverse-phase chromatographic performance. Chromatographic separation was performed on a MetaChem MonoChrom C-18 column (2.0 mm x 50 mm; 5 mum) at a flow rate of 1 mL/min. Compounds were eluted using a gradient mixture of water-acetic acid (100:0.1, v/v) and acetonitrile-acetic acid (100:0.1, v/v). The structural analog alpha-hydroxymethyltyrosine was used as the internal standard. Mass spectrometric detection was carried out with a triple quadrupole mass spectrometer. The method was validated and used to determine human plasma AMT concentrations, and has been implemented to derive pharmacokinetic parameters. (C) 2004 Elsevier B.V. All rights reserved.
引用
收藏
页码:229 / 234
页数:6
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