Determination of tramadol and metabolites by HPLC-FL and HPLC-MS/MS in urine of dogs

被引：43
作者：
Saccomanni, G. [2 ]
Del Carlo, S. [2 ]
Giorgi, M. [1 ]
Manera, C. [2 ]
Saba, A. [3 ]
Macchia, M. [2 ]
机构：
[1] Univ Pisa, Dept Vet Clin, I-56010 Pisa, Italy
[2] Univ Pisa, Dept Pharmaceut Sci, I-56126 Pisa, Italy
[3] Univ Pisa, Dept Chem & Ind Chem, I-56126 Pisa, Italy
来源：
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 2010年 / 53卷 / 02期
关键词：
Tramadol; Metabolites; Urine; HPLC-FL; HPLC-MS/MS; O-DESMETHYLTRAMADOL; ANALGESIC DRUG; HUMAN PLASMA; PHARMACOKINETICS; CHROMATOGRAPHY; HYDROCHLORIDE; M1; M5; M2;
D O I：
10.1016/j.jpba.2010.03.016
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Tramadol is a centrally acting analgesic drug used in veterinary and human clinical practice. Its metabolism has been largely characterized in human being but is still long to be comprehended in several animal species, especially in the dog. The aim of the present study was to develop and validate a new analytical procedure to investigate HPLC the metabolization/elimination process tramadol in urine of dogs by HPLC-FL or HPLC-MS/MS. A single oral dose of tramadol (4 mg/kg) was administered to 4 male Beagle dogs and the urine was naturally collected. This matrix either hydrolyzed than un-hydrolyzed was extracted with different blends of solvents to detect the total or free form of the analytes, respectively. The present method allowed to obtain good selectivity, accuracy, precision and recoveries without the need of time consuming or expensive clean up steps. The short chromatographic time courses allowed this analysis to be proposed for routine purposes. The HPLC-MS/MS detected in the urine two metabolites (M6 and M7) considered negligible in humans. The low LOQ showed that the method could be useful for the determination of the illegal use of this drug in race-dogs' urine. (C) 2010 Elsevier B.V. All rights reserved.
引用
收藏
页码：194 / 199
页数：6
相关论文
共 28 条
[1]   Both postnatal and postmenstrual age contribute to the interindividual variability in tramadol glucuronidation in neonates [J].
Allegaert, K. ;
Vanhole, C. ;
Vermeersch, S. ;
Rayyan, M. ;
Verbesselt, R. ;
de Hoon, J. .
EARLY HUMAN DEVELOPMENT, 2008, 84 (05) :325-330
[2]  
[Anonymous], CPMPICH38195
[3]   Improved liquid chromatographic method for the simultaneous determination of tramadol and its three main metabolites in human plasma, urine and saliva [J].
Ardakani, Yalda H. ;
Rouini, Mohammad-Reza .
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 2007, 44 (05) :1168-1173
[4]   Mechanistic aspects of electrospray ionization [J].
Bruins, AP .
JOURNAL OF CHROMATOGRAPHY A, 1998, 794 (1-2) :345-357
[5]   Enatiomeric determination of tramadol and O-desmethyltramadol in human urine by gas chromatography-mass spectrometry [J].
Chytil, Lukas ;
Sticha, Martin ;
Matouskova, Olga ;
Perlik, Frantisek ;
Slanar, Ondrej .
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2009, 877 (20-21) :1937-1942
[6]   Pharmacokinetics of tramadol and o-desmethyltramadol in goats after intravenous and oral administration [J].
De Sousa, A. B. ;
Santos, A. C. D. ;
Schramm, S. G. ;
Porta, V. ;
Gorniak, S. L. ;
Florio, J. C. ;
Spinosa, H. De Souza .
JOURNAL OF VETERINARY PHARMACOLOGY AND THERAPEUTICS, 2008, 31 (01) :45-51
[7]   The pharmacokinetics, metabolism and urinary detection time of tramadol in camels [J].
Elghazali, M. ;
Barezaik, I. M. ;
Hadi, A. A. Abdel ;
Eltayeb, F. M. ;
Al Masri, J. ;
Wasfi, I. A. .
VETERINARY JOURNAL, 2008, 178 (02) :272-277
[8]   Hydrolysis of conjugated metabolites of buprenorphine -: II.: The quantitative enzymatic hydrolysis of norbuprenorphine-3-β-D-glucuronide in human urine [J].
ElSohly, MA ;
Gul, W ;
Feng, SX ;
Murphy, TP .
JOURNAL OF ANALYTICAL TOXICOLOGY, 2005, 29 (06) :570-573
[9]   Pharmacokinetics of tramadol and its major metabolites following rectal and intravenous administration in dogs [J].
Giorgi, M. ;
Del Carlo, S. ;
Saccomanni, G. ;
Lebkowska-Wieruszewska, B. ;
Kowalski, C. J. .
NEW ZEALAND VETERINARY JOURNAL, 2009, 57 (03) :146-152
[10]   Pharmacokinetics of tramadol and its metabolites M1, M2 and M5 in horses following intravenous, immediate release (Fasted/Fed) and sustained release single dose administration [J].
Giorgi, Mario ;
Soldani, Giulio ;
Manera, Clementina ;
Ferrarini, PierLuigi ;
Sgorbini, Micaela ;
Saccomanni, Giuseppe .
JOURNAL OF EQUINE VETERINARY SCIENCE, 2007, 27 (11) :481-488
123