The interacting RNA polymerase II subunits, hRPB11 and hRPB3, are coordinately expressed in adult human tissues and down-regulated by doxorubicin

被引：18

作者：

Fanciulli, M ^{[1
]}

Bruno, T

Di Padova, M

De Angelis, R

Lovari, S

Floridi, A

Passananti, C

机构：

[1] Regina Elena Canc Inst, Cell Metab & Pharmacokinet Lab, I-00158 Rome, Italy

[2] Univ Aquila, Dept Expt Med, I-67100 Laquila, Italy

[3] CNR, Ist Tecnol Biomed, I-00137 Rome, Italy

来源：

FEBS LETTERS | 1998年 / 427卷 / 02期

关键词：

doxorubicin; drug toxicity; RNA polymerase II; alternative splicing; mRNA degradation; two-hybrid system;

D O I：

10.1016/S0014-5793(98)00431-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We previously isolated the human RPB11 cDN4, encoding the 13.3 kDa subunit of RNA polymerase II, and demonstrated that expression of this subunit is modulated by doxorubicin. Using hRPB11 as bait in a yeast two-hybrid system, two cDNA variants encoding a second RNA polymerase Ii subunit, hRPB3, have now been isolated and characterized. These two hRPB3 mRNA species differed in 3' UTR region length, the longer transcript containing the AU-rich sequence motif that mediates mRNA degradation. Both hRPB11 and hRPB3 transcripts share a similar pattern of distribution in human adult tissues, with particularly high levels in both heart and skeletal muscle, and the expression of both is down-regulated by doxorubicin as found previously for the hRPB11 subunit, Taken together, these findings suggest that the interaction between hRPB3 and hRPB11 is fundamental for their function and that this heterodimer is involved in doxorubicin toxicity. (C) 1998 Federation of European Biochemical Societies.

引用

页码：236 / 240

页数：5