Force measurements on platelet surfaces with high spatial resolution under physiological conditions

被引：27
作者：
Lee, I
Marchant, RE
机构：
[1] Case Western Reserve Univ, Dept Biomed Engn, Cleveland, OH 44106 USA
[2] Case Western Reserve Univ, Dept Macromol Sci, Cleveland, OH 44106 USA
来源：
COLLOIDS AND SURFACES B-BIOINTERFACES | 2000年 / 19卷 / 04期
基金：
美国国家卫生研究院;
关键词：
atomic force microscopy; force measurements; human platelet;
D O I：
10.1016/S0927-7765(00)00144-2
中图分类号：
Q6 [生物物理学];
学科分类号：
071011 ;
摘要：
Investigations on platelets are essential to understanding the regulation of hemostasis and thrombosis. Activated platelets undergo dramatic conformational and morphological changes mediated by numerous plasma proteins. AFM techniques can combine high spatial resolution with measurements of the mechanical properties of platelet surfaces. Here, we demonstrate two-dimensional force mapping over a human platelet adsorbed on glass under physiological buffer. The best resolution of platelet membrane elasticity we obtained was at 15.6 x 15.6 nm(2) pixel(-1). In addition, quantitative information on platelet surface charge density was extracted from individual force curves with the aid of DLVO theory. (C) 2000 Elsevier Science B.V. All rights reserved.
引用
收藏
页码：357 / 365
页数：9
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