In vitro propagation of Clitoria ternatea L.: A rare medicinal plant

被引:0
作者
Pandeya, Krishna [2 ]
Tiwari, Kavindra Nath [1 ]
Singh, Jayanti [1 ]
Verma, Jay Prakash [1 ]
Dubey, Satya Deo [2 ]
机构
[1] Banaras Hindu Univ, Mahila Mahavidyalaya, Dept Bot, Varanasi 221005, Uttar Pradesh, India
[2] Banaras Hindu Univ, Dept Dravyaguna, Fac Ayurveda, Inst Med Sci, Varanasi 221005, Uttar Pradesh, India
来源
JOURNAL OF MEDICINAL PLANTS RESEARCH | 2010年 / 4卷 / 08期
关键词
Clitoria ternatea; aparajita; axillary shoot proliferation; SOMATIC EMBRYOGENESIS; MICROPROPAGATION; REGENERATION; ORGANOGENESIS; ANTHOCYANINS;
D O I
暂无
中图分类号
R914 [药物化学];
学科分类号
100701 ;
摘要
A rapid micro-propagation protocol was established for rare medicinal plant Clitoria ternatea L. Multiple shoot formation was induced from shoot tip, node and cotyledonary node explants on Murashige and Skoog (MS) medium containing 2.0 mg/l BAP. Nodal explants gave the largest number (11.1) of shoots followed by cotyledonary node and shoot tip. MS medium containing 0.5 mg/l GA(3) was found most suitable for shoot elongation. Ex vitro rooting was achieved when the basal cut ends of elongated shoots were dipped in 250 mg/l IBA solution for half an hour followed by transplantation in plastic cups containing sterilized soilrite. The acclimatized plantlets were successfully established in the field with 90% survival rate. The plantlets are growing well without any phenotypic aberrations.
引用
收藏
页码:664 / 668
页数:5
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