Rapid cloning and expression of a fungal polyketide synthase gene involved in squalestatin biosynthesis

被引：60

作者：

Cox, RJ

Glod, F

Hurley, D

Lazarus, CM

Nicholson, TP

Rudd, BAM

Simpson, TJ

Wilkinson, B

Zhang, Y

机构：

[1] Univ Bristol, Sch Chem, Bristol BS8 1TS, Avon, England

[2] Univ Bristol, Sch Biol Sci, Bristol BS8 1UG, Avon, England

[3] GlaxoSmithKline, Stevenage SG1 2NY, Herts, England

来源：

CHEMICAL COMMUNICATIONS | 2004年 / 20期

关键词：

D O I：

10.1039/b411973h

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

PCR primers designed to selectively amplify the unique C-methyltransferase domain of fungal Polyketide synthases were used to selectively clone a polyketide synthase gene involved in the biosynthesis of the squalene synthase inhibitor squalestatin S1 1, heterologous expression of which led to the biosynthesis of the squalestatin side-chain.

引用

页码：2260 / 2261

页数：2

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