Antibacterial Effect of Oregano Essential Oil against Vibrio vulnificus and Its Mechanism

被引：46

作者：

Luo, Kunyao ^{[1
]}

Zhao, Pengyu ^{[1
]}

He, Yifei ^{[1
]}

Kang, Shengnan ^{[1
]}

Shen, Chenyu ^{[1
]}

Wang, Shuo ^{[1
]}

Guo, Meixian ^{[1
]}

Wang, Lehui ^{[1
]}

Shi, Chao ^{[1
]}

机构：

[1] Northwest A&F Univ, Coll Food Sci & Engn, 20 Xinong Rd, Xianyang 712100, Shaanxi, Peoples R China

来源：

FOODS | 2022年 / 11卷 / 03期

基金：

中国国家自然科学基金;

关键词：

antimicrobial activity; oregano essential oil; Vibrio vulnificus; membrane damage; reactive oxygen species; comet assay; VULGARE ESSENTIAL OIL; ANTIMICROBIAL ACTIVITY;

D O I：

10.3390/foods11030403

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Oregano essential oil (OEO) is an effective natural antibacterial agent, but its antibacterial activity against Vibrio vulnificus has not been widely studied. The aim of this study was to investigate the inhibitory effect and germicidal activity of OEO on V. vulnificus and its possible inhibition mechanism. The minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs) of OEO against four V. vulnificus strains (ATCC 27562 and three isolates from seafoods) were from 0.06 to 0.15 mu L/mL. Compared with untreated bacteria, OEO reduced the concentration of intracellular adenosine triphosphate (ATP), hyperpolarized the cell membrane, increased the level of reactive oxygen species (ROS), and increased the concentration of intracellular malondialdehyde (MDA), but there was no obvious DNA damage at the OEO test concentration. It was indicated that OEO inactivated V. vulnificus by generating ROS which caused lipid peroxidation of cell membranes, thereby reducing the permeability and integrity of cell membranes and causing morphological changes to cells, but there was no obvious damage to DNA. In addition, OEO could effectively kill V. vulnificus in oysters at 25 degrees C, and the number of bacteria decreased by 48.2% after 0.09% OEO treatment for 10 h. The good inhibitory effect and bactericidal activity of OEO showed in this study, and the economy and security of OEO make it possible to apply OEO to control V. vulnificus contamination in oysters and other seafoods.

引用

页数：17

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