Taurine Promotes the Cartilaginous Differentiation of Human Umbilical Cord-Derived Mesenchymal Stem Cells in Vitro

被引：6

作者：

Yao, Xiuhua ^{[1
]}

Huang, Huiling ^{[1
]}

Li, Zhou ^{[2
]}

Liu, Xiaohua ^{[3
]}

Fan, Weijia ^{[1
]}

Wang, Xinping ^{[4
]}

Sun, Xuelian ^{[3
]}

Zhu, Jianmin ^{[3
]}

Zhou, Hongrui ^{[2
]}

Wei, Huaying ^{[3
]}

机构：

[1] Tianjin Huanhu Hosp, Tianjin Neurosurg Inst, Tianjin Key Lab Cerebral Vasc & Neurodegenerat Di, 6 JiZhao Rd, Tianjin 300350, Peoples R China

[2] NewScen Coast Biopharmaceut Co Ltd, TEDA, 65 Sixth Ave, Tianjin 300457, Peoples R China

[3] Arthro Anda Tianjin Biol Technol Co Ltd, 2F Bldg 2,Tian Bao Ind Pk,Xi Qi Rd, Tianjin 300308, Peoples R China

[4] Tianjin Huanhu Hosp, Dept Neurol, 6 JiZhao Rd, Tianjin 300350, Peoples R China

来源：

NEUROCHEMICAL RESEARCH | 2017年 / 42卷 / 08期

基金：

国家高技术研究发展计划(863计划);

关键词：

Human mesenchymal stem cells; Umbilical cord; Taurine; Chondrogenesis; HUMAN ARTICULAR CHONDROCYTES; TISSUE GROWTH-FACTOR; PROGENITOR CELLS; BONE-MARROW; CHONDROGENIC DIFFERENTIATION; STIMULATES PROLIFERATION; PRIMARY CULTURES; EXPRESSION; OSTEOBLAST; PATHWAY;

D O I：

10.1007/s11064-017-2252-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Taurine has been reported to influence osteogenic differentiation, but the role of taurine on cartilaginous differentiation using human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) remains unclear. In this study, we investigated the effect of taurine (0, 1, 5 and 10 mM) on the proliferation and chondrogenesis of hUC-MSCs by analyzing cell proliferation, accumulation of glycosaminoglycans and expression of cartilage specific mRNA. The results show though taurine did not affected the proliferation of hUC-MSCs, 5 mM of taurine is sufficient to enhanced the accumulation of glycosaminoglycans and up-regulate cartilage specific mRNA expression, namely collagen type II, aggrecan and SOX9. Taurine also inhibits chondrocyte dedifferentiation by reducing expression of collagen type I mRNA. Taken together, our study reveals that taurine promotes and maintains the chondrogenesis of hUC-MSCs.

引用

页码：2344 / 2353

页数：10

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