Salvia miltiorrhiza;
Lamiaceae;
diterpenoids;
tanshinones;
HPLC-DAD-MS;
MAO A inhibition;
inhibition of iNOS induction;
RAW;
267.4;
cells;
D O I:
10.1055/s-2004-832615
中图分类号:
Q94 [植物学];
学科分类号:
071001 ;
摘要:
In the course of a screening of plant extracts for potential CNS and anti-inflammatory activities, a dichloromethane extract of Salvia miltiorrhiza showed a pronounced inhibitory effect on recombinant monoamine oxidase A (MAO A) and on inducible NO synthase (NOS) induction in Raw 267.4 cells. With the aid of HPLC-based profiling techniques, activities could be linked, to a significant extent, to peaks of tanshinone-type diterpenoids, four of which were subsequently isolated. The IC50 of the most active compound, 15,16-dihydrotanshinone 1, on human recombinant MAO A was at 23 muM, and 2.4 muM on lipopolysaccharide-mediated iNOS induction in Raw 267.4 cells.