Structure of immature West Nile virus

被引：105

作者：

Zhang, Ying ^{[1
]}

Kaufmann, Barbel ^{[1
]}

Chipman, Paul R. ^{[1
]}

Kuhn, Richard J. ^{[1
]}

Rossmann, Michael G. ^{[1
]}

机构：

[1] Purdue Univ, Dept Biol Sci, W Lafayette, IN 47907 USA

来源：

JOURNAL OF VIROLOGY | 2007年 / 81卷 / 11期

关键词：

D O I：

10.1128/JVI.00037-07

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The structure of immature West Nile virus particles, propagated in the presence of ammonium chloride to block virus maturation in the low-pH environment of the trans-Golgi network, was determined by cryo-electron microscopy (cryo-EM). The structure of these particles was similar to that of immature West Nile virus particles found as a minor component of mature virus samples (naturally occurring immature particles [NOIP's]). The structures of mature infectious flaviviruses are radically different from those of the immature particles. The similarity of the ammonium chloride-treated particles and NOIPs suggests either that the NOIPs have not undergone any conformational change during maturation or that the conformational change is reversible. Comparison with the cryo-EM reconstruction of immature dengue virus established the locations of the N-linked glycosylation sites of these viruses, verifying the interpretation of the reconstructions of the immature flaviviruses.

引用

页码：6141 / 6145

页数：5

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