Fourier transform infrared microspectroscopy identifies early lineage commitment in differentiating human embryonic stem cells

被引：56

作者：

Heraud, Philip ^{[1
,2
,3
]}

Ng, Elizabeth S. ^{[1
]}

Caine, Sally ^{[1
,2
,3
]}

Yu, Qing C. ^{[1
]}

Hirst, Claire ^{[1
]}

Mayberry, Robyn ^{[1
]}

Bruce, Amanda ^{[1
]}

Wood, Bayden R. ^{[2
,3
]}

McNaughton, Don

Stanley, Edouard G. ^{[1
]}

Elefanty, Andrew G. ^{[1
]}

机构：

[1] Monash Univ, Monash Immunol & Stem Cell Labs, Clayton, Vic 3800, Australia

[2] Monash Univ, Ctr Biospect, Clayton, Vic 3800, Australia

[3] Monash Univ, Sch Chem, Clayton, Vic 3800, Australia

来源：

STEM CELL RESEARCH | 2010年 / 4卷 / 02期

基金：

英国医学研究理事会;

关键词：

FT-IR SPECTROSCOPY; LIGHT;

D O I：

10.1016/j.scr.2009.11.002

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Human ESCs (hESCs) are a valuable tool for the study of early human development and represent a source of normal differentiated cells for pharmaceutical and biotechnology applications and ultimately for cell replacement therapies. For all applications, it will be necessary to develop assays to validate the efficacy of hESC differentiation. We explored the capacity for FTIR spectroscopy, a technique that rapidly characterises cellular macromolecular composition, to discriminate mesendoderm or ectoderm committed cells from undifferentiated hESCs. Distinct infrared spectroscopic "signatures" readily distinguished hESCs from these early differentiated progeny, with bioinformatic models able to correctly classify over 97% of spectra. These data identify a role for FTIR spectroscopy as a new modality to complement conventional analyses of hESCs and their derivatives. FTIR spectroscopy has the potential to provide low-cost, automatable measurements for the quality control of stem and differentiated cells to be used in industry and regenerative medicine. Crown Copyright (C) 2009 Published by Elsevier B.V. All rights reserved.

引用

页码：140 / 147

页数：8

共 20 条

[1] Embryonic stem cell differentiation studied by FT-IR spectroscopy [J].

Ami, Diletta ;

Neri, Tui ;

Natalello, Antonino ;

Mereghetti, Paolo ;

Doglia, Silvia Maria ;

Zanoni, Mario ;

Zuccotti, Maurizio ;

Garagna, Silvia ;

Redi, Carlo Alberto .

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH, 2008, 1783 (01) :98-106

[2]

[Anonymous], 2003, Sage, Thousand Oaks

[3]

Chalmers J.M., 2006, Polymorphism in the pharmaceutical industry

[4]

Costa M, 2008, CURR PROTOC STEM CEL

[5] Targeting a GFP reporter gene to the MIXL1 locus of human embryonic stem cells identifies human primitive streak-like cells and enables isolation of primitive hematopoietic precursors [J].

Davis, Richard P. ;

Ng, Elizabeth S. ;

Costa, Magdaline ;

Mossman, Anna K. ;

Sourris, Koula ;

Elefanty, Andrew G. ;

Stanley, Edouard G. .

BLOOD, 2008, 111 (04) :1876-1884

[6] BMP signalling inhibits premature neural differentiation in the mouse embryo [J].

Di-Gregorio, Aida ;

Sancho, Margarida ;

Stuckey, Daniel W. ;

Crompton, Lucy A. ;

Godwin, Jonathan ;

Mishina, Yuji ;

Rodriguez, Tristan A. .

DEVELOPMENT, 2007, 134 (18) :3359-3369

[7] Infrared spectroscopy of cells and tissues: Shining light onto a novel subject [J].

Diem, M ;

Boydston-White, S ;

Chiriboga, L .

APPLIED SPECTROSCOPY, 1999, 53 (04) :148A-161A

[8]

Geladi P., 1988, J CHEMOM, V2, P231

[9] Effects of pre-processing of Raman spectra on in vivo classification of nutrient status of microalgal cells [J].

Heraud, Philip ;

Wood, Bayden R. ;

Beardall, John ;

McNaughton, Don .

JOURNAL OF CHEMOMETRICS, 2006, 20 (05) :193-197

[10] MULTILAYER FEEDFORWARD NETWORKS ARE UNIVERSAL APPROXIMATORS [J].

HORNIK, K ;

STINCHCOMBE, M ;

WHITE, H .

NEURAL NETWORKS, 1989, 2 (05) :359-366

← 1 2 →