Comparison of Point-of-Care Tests for the Rapid Diagnosis of Visceral Leishmaniasis in East African Patients

被引：0

作者：

Bezuneh, Asrat ^{[1
]}

Mukhtar, Maowia ^{[2
]}

Abdoun, Asim ^{[2
]}

Teferi, Tedla ^{[3
]}

Takele, Yegnasew ^{[4
]}

Diro, Ermias ^{[4
]}

Jemaneh, Asfaw ^{[3
]}

Shiferaw, Welelta ^{[1
]}

Wondimu, Hirut ^{[1
]}

Bhatia, Ajay ^{[5
]}

Howard, Randall F. ^{[6
]}

Ghalib, Hashim ^{[6
]}

Ireton, Gregory C. ^{[6
]}

Hailu, Asrat ^{[1
]}

Reed, Steven G. ^{[6
]}

机构：

[1] Univ Addis Ababa, Dept Microbiol Immunol & Parasitol, Addis Ababa, Ethiopia

[2] Univ Khartoum, Inst Endem Dis, Khartoum, Sudan

[3] Arba Minch Hosp, Leishmaniasis Res & Treatment Ctr, Sikela, Ethiopia

[4] Univ Gondar, Leishmaniasis Res & Treatment Ctr, Gondar, Ethiopia

[5] BioFire Diagnost LLC, Salt Lake City, UT USA

[6] Infect Dis Res Inst, Seattle, WA 98102 USA

来源：

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE | 2014年 / 91卷 / 06期

关键词：

LATEX AGGLUTINATION-TEST; DONOVANI INFECTIONS; TEST KATEX; ANTIGEN; SERODIAGNOSIS; INDIA; PCR;

D O I：

10.4269/ajtmh.14-0759

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

The development of rK39-based rapid diagnostic tests (RDTs) has greatly aided the diagnosis of visceral leishmaniasis, especially in the Indian subcontinent and Brazil, by offering high sensitivity and specificity. However, these tests have been less sensitive and less specific in sub-Saharan Africa. To improve upon the performance of rK39 in Africa, we engineered the fusion molecule rK28, which retained some of the rK39 repeats and combined them with repeat sequences from two additional Leishmania genes. This polyprotein was used in the development of several prototype RDTs by different commercial manufacturers with the goal of assessing relative performance in inexpensive formats. Here, we report field studies showing that The rK28 antigen could be readily adapted to a variety of RDT formats to achieve high sensitivity, generally > 90%, and adequate specificity to aid in the diagnosis of human visceral leishmaniasis in East Africa, Asia, and South America.

引用

页码：1109 / 1115

页数：7

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