Dried blood spot validation of five immunosuppressants, without hematocrit correction, on two LC-MS/MS systems

被引：45
作者：
Koster, Remco A. [1 ]
Veenhof, Herman [1 ]
Botma, Rixt [1 ]
Hoekstra, Alle Tjipke [1 ]
Berger, Stefan P. [2 ]
Bakker, Stephan J. L. [2 ]
Alffenaar, Jan-Willem C. [1 ]
Touw, Daan J. [1 ,3 ]
机构：
[1] Univ Groningen, Univ Med Ctr Groningen, Dept Clin Pharm & Pharmacol, Groningen, Netherlands
[2] Univ Groningen, Univ Med Ctr Groningen, Dept Internal Med, Div Nephrol, Groningen, Netherlands
[3] Univ Groningen, Sect Pharmacokinet Toxicol & Targeting, Dept Pharm, Groningen, Netherlands
来源：
BIOANALYSIS | 2017年 / 9卷 / 07期
关键词：
clinical validation; cyclosporine A; DBS; dried blood spot; everolimus; hematocrit effect; immunosuppressants; mycophenolic acid; sirolimus; tacrolimus; CLINICAL-CHEMISTRY; SPECTROSCOPY; PREDICTION;
D O I：
10.4155/bio-2016-0296
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Aim: Hematocrit (Ht) effects remain a challenge in dried blood spot (DBS) sampling. The aim was to develop an immunosuppressant DBS assay on two LC-MS/MS systems covering a clinically relevant Ht range without Ht correction. Results: The method was partially validated for tacrolimus, sirolimus, everolimus, cyclosporin A and fully validated for mycophenolic acid on an Agilent and Thermo LC-MS/MS system. Bias caused by Ht effects were within 15% for all immunosuppressants between Ht levels of 0.23 and 0.48 l/l. Clinical validation of DBS versus whole blood samples for tacrolimus and cyclosporin A showed no differences between the two matrices. Conclusion: A multiple immunosuppressant DBS method without Ht correction, has been validated, including a clinical validation for tacrolimus and cyclosporin A, making this procedure suitable for home sampling.
引用
收藏
页码：553 / 563
页数：11
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