Subpicosecond UV spectroscopy of carbonmonoxy-myoglobin: absorption and circular dichroism studies

被引：28

作者：

Dartigalongue, Thibault ^{[1
]}

Niezborala, Claire ^{[1
]}

Hache, Francois ^{[1
]}

机构：

[1] Ecole Polytech, CNRS, INSERM, Lab Opt & Biosci, F-91128 Palaiseau, France

来源：

PHYSICAL CHEMISTRY CHEMICAL PHYSICS | 2007年 / 9卷 / 13期

关键词：

D O I：

10.1039/b616173a

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

A thorough absorption and circular dichroism study is performed in carbonmonoxy-myoglobin with a sub-picosecond visible pump, ultraviolet probe experiment. Differential absorption in the 220-360 nm range shows that the time-resolved response mainly comes from the heme and that aromatic amino acids do not contribute significantly. Time-resolved CD at 260 nm shows no dynamics and confirms this result. On the contrary, a strong CD dynamics is observed at 230 nm. This signal could originate from transient deformation of the alpha-helices in the protein.

引用

页码：1611 / 1615

页数：5

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