Validation of HPLC determination of phenolic acids present in some Lamiaceae family plants

被引：43

作者：

Ziaková, A ^{[1
]}

Brandsteterová, E ^{[1
]}

机构：

[1] Slovak Univ Technol Bratislava, Fac Chem & Food Technol, Dept Analyt Chem, Bratislava 81237, Slovakia

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES | 2003年 / 26卷 / 03期

关键词：

validation; HPLC; phenolic acids; plants;

D O I：

10.1081/JLC-120017181

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

HPLC columns with different length, particle size, and chemical properties of sorbent were tested and compared for the application in the development of the universal HPLC assay for determination of phenolic compounds, which could be present in some medicinal plants from the Lamiaceae family (Melissa officinalis, Rosmarinus officinalis, Salvia officinalis, Thymus serpyllum, and Origanum vulgare). More RP-18 columns have been chosen for the simultaneous separation of all phenolic compounds in the study. The basic chromatographic characteristics were evaluated and the HPLC method using one of the suitable columns was validated for all determined analytes. The recommended separation conditions were applied for phenolic compound monitoring in extracts of plant material. Yields of analytes present in all plant samples were evaluated.

引用

页码：443 / 453

页数：11

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