Isolation of lactoferrin from acid whey by magnetic affinity separation

被引：48

作者：

Chen, Lin ^{[1
]}

Guo, Chen ^{[1
]}

Guan, Yueping ^{[1
]}

Liu, Huizhou ^{[1
]}

机构：

[1] Chinese Acad Sci, Inst Proc Engn, Lab Separat Sci & Engn, Beijing 100080, Peoples R China

来源：

SEPARATION AND PURIFICATION TECHNOLOGY | 2007年 / 56卷 / 02期

基金：

中国国家自然科学基金;

关键词：

lactoferrin; whey; magnetic affinity absorbents; PGMA;

D O I：

10.1016/j.seppur.2007.01.019

中图分类号：

TQ [化学工业];

学科分类号：

0817 ;

摘要：

A micron-sized monodisperse superparamagnetic polyglycidyl methacrylate (PGMA) particles coupled with heparin (PGMA-heparin) was synthesized by the polymerization, and then was evaluated as a method for purifying lactoferrin (LF) from bovine whey. The content of heparin groups at the surface of PGMA particles was 0.92 mg/g. Magnetic PGMA-heparin particles were highly effective in recovering LF directly from acid whey by a single step, whose purity was higher than that of commercial standard proteins, using NaCl solutions as eluent. The maximum LF binding capacity was 164 mg/g. The competitive adsorption tests verified that the magnetic approach offered a fast process and potential at a large scale. The practical use of magnetic PGMA-heparin particles for production of high purified LF from whey is a realistic prospect. (c) 2007 Elsevier B.V. All rights reserved.

引用

页码：168 / 174

页数：7

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