Transcription Factor Binding and Nucleosome Positioning Are Alternative Pathways for Transcription Start Site Selection in Eukaryotic Promoters

被引：0

作者：

Dreos, Rene ^{[1
]}

Ambrosini, Giovanna ^{[1
]}

Bucher, Philipp ^{[1
]}

机构：

[1] Swiss Inst Bioinformat, CH-1015 Lausanne, Switzerland

来源：

PROCEEDINGS IWBBIO 2014: INTERNATIONAL WORK-CONFERENCE ON BIOINFORMATICS AND BIOMEDICAL ENGINEERING, VOLS 1 AND 2 | 2014年

关键词：

transcription start site; nucleosome; promoters; TATAbox; CORE PROMOTERS; GENOME-WIDE; TATA-BOX; CHROMATIN ORGANIZATION; GENE-EXPRESSION; HUMAN-CELLS; YEAST; DNA; IDENTIFICATION; DETERMINANTS;

D O I：

暂无

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

The DNA sequence determinants which direct RNA Pol-II to the correct transcription start site (TSS) are only partly understood. Conserved DNA motifs (core promoter elements) or a conserved nucleosome architecture may play a role in TSS selection. A complicating factor is that promoters are quite variable in many respects. Some have very focused while others have highly dispersed initiation site patters. Promoters also differ by the presence or absence of CPEs. Here we show that promoters without CPEs have a strong sequence-intrinsic nucleosomepositioning signal in the +1 nucleosome region, in both vertebrates and flies. The strength of the signal is inversely proportional to the degree of TSS dispersion. Interestingly, the nucleosome-positioning signal is completely absent in CPE containing promoters. Together, these findings suggest that transcription factor binding to CPEs and DNA sequenceinduced nucleosome positioning are two mutually exclusive pathways of Pol-II recruitment to TSSs in eukaryotic promoters.

引用

页码：695 / 706

页数：12

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