TGF-β1 overexpression in the transversalis fascia of patients with direct inguinal hernia

被引:19
作者
Pascual, G. [1 ]
Corrales, C. [1 ]
Gomez-Gil, V. [1 ]
Bujan, J. [1 ]
Bellon, J. M. [1 ]
机构
[1] Univ Alcala, Ctr Madrid Barcelona, Fac Med, Dept Surg, Madrid 28871, Spain
关键词
extracellular matrix; growth factors; hernia repair; inguinal hernia; metalloproteinases; TGF beta; transversalis fascia;
D O I
10.1111/j.1365-2362.2007.01816.x
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Background The aetiology of inguinal hernia includes changes in collagen turnover and metalloproteinase (MMP) expression, and direct hernia has been linked to increased MMP-2 expression. Since transforming growth factor beta 1 (TGF beta 1) plays a role in tissue remodelling, this growth factor could directly affect metalloproteinase secretion and thus the proteolytic activity of these enzymes. We hypothesized that TGF beta 1 expression could also be altered in direct inguinal hernias. Materials and methods Tissue specimens were obtained from the transversalis fascia (TF) of organ donors (controls; n = 10) and patients with inguinal hernia (indirect; n = 20/direct; n = 20), who were also divided into two groups according to age (20-40/41-60 years). Tissue sections were immunohistochemically labelled using anti-LAP TGF beta 1 (latent form) and anti-TGF beta 1 (active form) antibodies, and fragments of tissue were subjected to Western blot analysis. Results No significant differences in LAP-TGF beta 1 expression were detected between specimens from control and hernia patients. However, significantly higher levels of active TGF beta 1 were detected in the TF of patients with direct hernia (P < 0.05). Age affected the expression of the growth factor in its active form, and significant differences emerged between direct hernias and controls/indirect hernias only in the younger age groups. Conclusions Our findings indicate overexpression of the active form of TGF beta 1 in the TF of young patients with direct hernia. This overexpression reflects an attempt to counterbalance the enhanced matrix degradation process observed in these patients, identifying a subset of patients requiring the use of a prosthetic material for primary hernia repair.
引用
收藏
页码:516 / 521
页数:6
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