TGF-β1 overexpression in the transversalis fascia of patients with direct inguinal hernia

被引:19
作者
Pascual, G. [1 ]
Corrales, C. [1 ]
Gomez-Gil, V. [1 ]
Bujan, J. [1 ]
Bellon, J. M. [1 ]
机构
[1] Univ Alcala, Ctr Madrid Barcelona, Fac Med, Dept Surg, Madrid 28871, Spain
关键词
extracellular matrix; growth factors; hernia repair; inguinal hernia; metalloproteinases; TGF beta; transversalis fascia;
D O I
10.1111/j.1365-2362.2007.01816.x
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Background The aetiology of inguinal hernia includes changes in collagen turnover and metalloproteinase (MMP) expression, and direct hernia has been linked to increased MMP-2 expression. Since transforming growth factor beta 1 (TGF beta 1) plays a role in tissue remodelling, this growth factor could directly affect metalloproteinase secretion and thus the proteolytic activity of these enzymes. We hypothesized that TGF beta 1 expression could also be altered in direct inguinal hernias. Materials and methods Tissue specimens were obtained from the transversalis fascia (TF) of organ donors (controls; n = 10) and patients with inguinal hernia (indirect; n = 20/direct; n = 20), who were also divided into two groups according to age (20-40/41-60 years). Tissue sections were immunohistochemically labelled using anti-LAP TGF beta 1 (latent form) and anti-TGF beta 1 (active form) antibodies, and fragments of tissue were subjected to Western blot analysis. Results No significant differences in LAP-TGF beta 1 expression were detected between specimens from control and hernia patients. However, significantly higher levels of active TGF beta 1 were detected in the TF of patients with direct hernia (P < 0.05). Age affected the expression of the growth factor in its active form, and significant differences emerged between direct hernias and controls/indirect hernias only in the younger age groups. Conclusions Our findings indicate overexpression of the active form of TGF beta 1 in the TF of young patients with direct hernia. This overexpression reflects an attempt to counterbalance the enhanced matrix degradation process observed in these patients, identifying a subset of patients requiring the use of a prosthetic material for primary hernia repair.
引用
收藏
页码:516 / 521
页数:6
相关论文
共 50 条
  • [21] Overexpression of transforming growth factor (TGF)-β1 and TGF-β3 genes in lung of toxic-inhaled patients
    Zarin, Aref Arzan
    Behmanesh, Mehrdad
    Tavallaei, Mahmoud
    Shohrati, Majid
    Ghanei, Mostafa
    EXPERIMENTAL LUNG RESEARCH, 2010, 36 (05) : 284 - 291
  • [22] Active matrix metalloproteinase-2 upregulation in the abdominal skin of patients with direct inguinal hernia
    Pascual, Gemma
    Rodriguez, Marta
    Gomez-Gil, Veronica
    Trejo, Cynthia
    Bujan, Julia
    Manvel Bellon, Juan
    EUROPEAN JOURNAL OF CLINICAL INVESTIGATION, 2010, 40 (12) : 1113 - 1121
  • [23] Overexpression of latent transforming growth factor-β1 (TGF-β1) binding protein 1 (LTBP-1) in association with TGF-β1 in ovarian carcinoma
    Higashi, T
    Sasagawa, T
    Inoue, M
    Oka, R
    Leng, SY
    Saijoh, K
    JAPANESE JOURNAL OF CANCER RESEARCH, 2001, 92 (05): : 506 - 515
  • [24] TGF-β1 in patients with renal cell carcinoma
    Hegele, A
    Varga, Z
    von Knobloch, R
    Heidenreich, A
    Kropf, J
    Hofmann, R
    UROLOGICAL RESEARCH, 2002, 30 (02): : 126 - 129
  • [25] Early and adult hippocampal TGF-β1 overexpression have opposite effects on behavior
    Depino, Amaicha Mara
    Lucchina, Luciana
    Pitossi, Fernando
    BRAIN BEHAVIOR AND IMMUNITY, 2011, 25 (08) : 1582 - 1591
  • [26] Disruption of anterior segment development by TGF-β1 overexpression in the eyes of transgenic mice
    Flügel-Koch, C
    Ohlmann, A
    Platigorsky, J
    Tamm, ER
    DEVELOPMENTAL DYNAMICS, 2002, 225 (02) : 111 - 125
  • [27] Status of higher TGF-β1 and TGF-β2 levels in the aqueous humour of patients with diabetes and cataracts
    Gao, Chao
    Lin, Xiaolei
    Fan, Fan
    Liu, Xin
    Wan, Huijuan
    Yuan, Ting
    Zhao, Xinrong
    Luo, Yi
    BMC OPHTHALMOLOGY, 2022, 22 (01)
  • [28] Surfactant dysfunction during overexpression of TGF-β1 precedes profibrotic lung remodeling in vivo
    Lopez-Rodriguez, Elena
    Boden, Caroline
    Echaide, Mercedes
    Perez-Gil, Jesus
    Kolb, Martin
    Gauldie, Jack
    Maus, Ulrich A.
    Ochs, Matthias
    Knudsen, Lars
    AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY, 2016, 310 (11) : L1260 - L1271
  • [29] Changes of Plasma uPA and TGF-β1 in Patients with Preeclampsia
    Fan, Minghua
    Xu, Yongping
    Qiu, Jianqing
    2016 3RD INTERNATIONAL SYMPOSIUM ON ENGINEERING TECHNOLOGY, EDUCATION AND MANAGEMENT (ISETEM 2016), 2016, : 144 - 148
  • [30] Increased expression of Ob-Rb and its relationship with the overexpression of TGF-β1 and the stage of fibrosis in patients with nonalcoholic steatohepatitis
    Cayon, A.
    Crespo, Javier
    Mayorga, M.
    Guerra, A.
    Pons-Romero, F.
    LIVER INTERNATIONAL, 2006, 26 (09) : 1065 - 1071