PURIFICATION OF PHENOLIC COMPOUNDS FROM Inga edulis LEAVES USING SOLID-PHASE EXTRACTION: MAJOR COMPOUNDS QUANTIFICATION AND ANTIOXIDANT CAPACITY EVALUATION

PURIFICATION OF PHENOLIC COMPOUNDS FROM Inga edulis LEAVES USING SOLID-PHASE EXTRACTION: MAJOR COMPOUNDS QUANTIFICATION AND ANTIOXIDANT CAPACITY EVALUATION. A phenolic fraction was obtained from of the acetone-water-acetic acid extract of Inga edulis leaves, by liquid-liquid partition and SPE-C-18 cartridges. This method provided an increase of 108, 66, 51, 50 and 36% of flavonols, proanthocyanidins, total polyphenols, gallotannins and flavanols, respectively. The major phenolics in purified fraction were procyanidin B2, catechin and myricetin-3-O-alpha-L-rhamnopyranoside, which achieved increases of 111, 47 and 45%, respectively, after SPE. Acid hydrolysis confirmed the presence of procyanidins, prodelphinidins and glycosylated flavonoids.