Molecular cloning and characterization of LPS-binding protein/bactericidal permeability-increasing protein (LBP/BPI) from olive flounder, Paralichthys olivaceus

被引:17
|
作者
Nam, Bo-Hye [1 ]
Ahn, Kyoung Jin [1 ]
Kim, Young-Ok [1 ]
Kong, Hee Jeong [1 ]
Kim, Woo-Jin [1 ]
Kim, Hyung Soo [1 ]
Lee, Sang-Jun [1 ]
Kim, Kyong Kil [1 ]
机构
[1] Natl Fisheries Res & Dev Inst, Aquaculture Ind Dept, Biotechnol Res Div, Pusan 619902, South Korea
关键词
LPS; LBP/BPI; Olive flounder; Paralichthys olivaceus; GADUS-MORHUA L; SEQUENCE ALIGNMENT; HOST-DEFENSE; LIPOPOLYSACCHARIDE; BPI; EXPRESSION; BPI/LBP; GENE; ENDOTOXIN; RECEPTOR;
D O I
10.1016/j.vetimm.2009.07.010
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
A lipopolysaccharide (LPS)-binding protein/bactericidal permeability-increasing protein (LBP/BPI) homolog was isolated from peripheral blood leukocytes cDNA library of olive flounder Paralichthys olivaceus. The isolated LBP/BPI cDNA is 2806 bp in length with a 1419 bp open reading frame (ORF) that encodes a protein of 472 amino acid residues. The LPS-binding domain is well conserved in the N-terminal barrel, showing high sequence identities with other teleost LBP/BPI as well as those of mammals. RT-PCR analysis revealed that mRNA expression of LBP/BPI was significantly elevated in all tested tissues (liver, gill, intestine, head kidney, and spleen) after intraperitoneal injection of the gram-negative bacterium Edwardsiella tarda or the gram-positive bacterium Streptococcus iniae. This expression pattern profile corresponded to that of acute inflammatory cytokines, suggesting that it plays a role in the innate immune response, in particular, the acute phase response. (C) 2009 Elsevier B.V. All rights reserved.
引用
收藏
页码:256 / 263
页数:8
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