Determination of serum albumin in the presence of poly(diallyldimethylammonium chloride) by resonance light scattering technique

By means of the resonance light scattering (RLS) technique, a new method was developed to determine the bovine serum albumin (BSA) and human serum albumin (HSA) by the interaction of serum albumin with poly(diallyldimethylammonium chloride) (PDDA). At Tris-NaOH buffer solution, the RLS intensity of serum albumin at the wavelength 320, 550 and 590nm was obviously enhanced in the presence of PDDA. The influences of some experimental factors, including incubation time, addition sequence of reagents, pH value, concentration of PDDA and foreign substances, on the enhancement of the RLS intensity were examined. The optimum conditions of the experiment were selected. Under the selected experimental condition, the enhanced RLS intensities were directly proportional to the concentrations in the range of (0.0250-2.75) x 10(-6) mol/L for BSA and (0.0235-1.17) x 10(-6) mol/L for HSA. The detection limits (S/N = 3) were 8.40 x 10(-9) mol/L for BSA and 7.39 x 10(-9) mol/L for HSA. The synthetic samples were analysed and the results obtained were satisfactory. (c) 2006 Elsevier B.V. All rights reserved.