Polymeric immunoglobulin receptor gene of mouse: sequence, structure and chromosomal location

We have isolated genomic clones of a mouse gene (pIgR) for polymeric immunoglobulin receptor which mediates transport of polymeric immunoglobulins. The four overlapping clones obtained retain a DNA fragment spanning approx. 32 kb altogether, and the base sequences of these clones were determined. Comparison with cDNA sequence identified 11 exons and 10 introns, as well as a polyadenylation site. We have also identified presumptive regulatory elements on the 5' presumptive untranscribed region and a polyadenylation signal on the 3' untranslated region. Thus, the DNA cloned covers the whole area which is transcribed into mRNA. Also, in situ hybridization locates this gene on the long arm of the first chromosome of mouse. (C) 1997 Elsevier Science B.V.