Estrogenic activities of two synthetic pyrethroids and their metabolites

被引:50
作者
Jin, Meiqing [2 ]
Li, Ling [1 ]
Xu, Chao [1 ]
Wen, Yuezhong [2 ]
Zhao, Meirong [1 ]
机构
[1] Zhejiang Univ Technol, Coll Biol & Environm Engn, Res Ctr Environm Sci, Hangzhou 310032, Zhejiang, Peoples R China
[2] Zhejiang Univ, Coll Environm & Resource Sci, MOE Key Lab Environm Remediat & Ecosyst Hlth, Hangzhou 310027, Peoples R China
关键词
pyrethroids; metabolites; cell proliferation; gene expression; estrogenic activity; REPORTER GENE ASSAY; IN-VITRO ASSAYS; CELL-LINE; INSECTICIDE BIFENTHRIN; DISRUPTING CHEMICALS; LAMBDA-CYHALOTHRIN; RECEPTOR; ENANTIOSELECTIVITY; SENSITIVITY; PESTICIDES;
D O I
10.1016/S1001-0742(09)60107-8
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
Synthetic pyrethroids (SPs) are among the most common pesticides in current use, and so far, several SPs have been assessed for their potential estrogenicities by various methods. Previous studies have shown that the estrogenicities partly come from their metabolites. Although considerable information is available with respect to the metabolism and environmental degradation of SPs, little is known about the estrogenicities of the metabolites. In this study, permethrin (PM) and beta-cypermethrin (CP), as well as their metabolites (3-phenoxybenzoic alcohol (PBCOH), 3-phenoxybenzaldehyde (PBCHO) and 3-phenoxybenzoic acid (PBCOOH) were evaluated for their estrogenic activities in the MCF-7 human breast carcinoma cell line. In the MCF-7 cell proliferation assay, PM and CP exhibited significant estrogenic activities at 10(-7) mol/L, comparable to 17 beta-estradiol (E-2) of 10(-9) mol/L, with the relative proliferative effect ratios of 55.4% and 56.3%, respectively. The real-time quantitative polymerase chain reaction (qRT-PCR) results confirmed the estrogenicities of PM and CP with significant alteration of pS2 and ER alpha mRNA levels observed at 10(-6) mol/L. For the three major metabolites, PBCOH and PBCOOH exhibited estrogenic activities in all assays, while no significant estrogenic responses was observed for PBCHO compared to the vehicle control. In particular, PBCOH had even slightly stronger estrogenic activity than its parent compounds, indicating that metabolism may be one of the reasons for the estrogenicities of the SPs. Given the widespread use of SPs, the toxicological effects of parent compounds and their metabolites should be taken into consideration in the risk assessment of SPs.
引用
收藏
页码:290 / 296
页数:7
相关论文
共 32 条
[1]   Comparison of short-term estrogenicity tests for identification of hormone-disrupting chemicals [J].
Andersen, HR ;
Andersson, AM ;
Arnold, SF ;
Autrup, H ;
Barfoed, M ;
Beresford, NA ;
Bjerregaard, P ;
Christiansen, LB ;
Gissel, B ;
Hummel, R ;
Jorgensen, EB ;
Korsgaard, B ;
Le Guevel, R ;
Leffers, H ;
McLachlan, J ;
Moller, A ;
Nielsen, JB ;
Olea, N ;
Oles-Karasko, A ;
Pakdel, F ;
Pedersen, KL ;
Perez, P ;
Skakkeboek, NE ;
Sonnenschein, C ;
Soto, AM ;
Sumpter, JP ;
Thorpe, SM ;
Grandjean, P .
ENVIRONMENTAL HEALTH PERSPECTIVES, 1999, 107 :89-108
[2]   PYRETHRUM FLOWERS AND PYRETHROID INSECTICIDES [J].
CASIDA, JE .
ENVIRONMENTAL HEALTH PERSPECTIVES, 1980, 34 (FEB) :189-202
[3]   DEVELOPMENTAL EFFECTS OF ENDOCRINE-DISRUPTING CHEMICALS IN WILDLIFE AND HUMANS [J].
COLBORN, T ;
SAAL, FSV ;
SOTO, AM .
ENVIRONMENTAL HEALTH PERSPECTIVES, 1993, 101 (05) :378-384
[4]   ENVIRONMENTAL ESTROGENS - HEALTH IMPLICATIONS FOR HUMANS AND WILDLIFE [J].
COLBORN, T .
ENVIRONMENTAL HEALTH PERSPECTIVES, 1995, 103 :135-136
[5]   Modes of action in ecotoxicology: Their role in body burdens, species sensitivity, QSARs, and mixture effects [J].
Escher, BI ;
Hermens, JLM .
ENVIRONMENTAL SCIENCE & TECHNOLOGY, 2002, 36 (20) :4201-4217
[6]   Quantitative comparisons of in vitro assays for estrogenic activities [J].
Fang, H ;
Tong, WD ;
Perkins, R ;
Soto, AM ;
Prechtl, NV ;
Sheehan, DM .
ENVIRONMENTAL HEALTH PERSPECTIVES, 2000, 108 (08) :723-729
[7]   Estrogenic and antiprogestagenic activities of pyrethroid insecticides [J].
Garey, J ;
Wolff, MS .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1998, 251 (03) :855-859
[8]   DIFFERENTIAL TOXICITY OF TRANS-PERMETHRIN IN RAINBOW-TROUT AND MICE .2. ROLE OF TARGET ORGAN SENSITIVITY [J].
GLICKMAN, AH ;
LECH, JJ .
TOXICOLOGY AND APPLIED PHARMACOLOGY, 1982, 66 (02) :162-171
[9]   Estrogenic potential of certain pyrethroid compounds in the MCF-7 human breast carcinoma cell line [J].
Go, V ;
Garey, J ;
Wolff, MS ;
Pogo, BGT .
ENVIRONMENTAL HEALTH PERSPECTIVES, 1999, 107 (03) :173-177
[10]   Metabolites of pyrethroid insecticides in urine specimens: Current exposure in an urban population in Germany [J].
Heudorf, U ;
Angerer, J .
ENVIRONMENTAL HEALTH PERSPECTIVES, 2001, 109 (03) :213-217