Effect of NaCl on leaf H+-ATPase and the relevance to salt tolerance in two contrasting poplar species

被引:18
作者
Ma, Xiuying [1 ]
Deng, Lin [1 ]
Li, Jinke [1 ]
Zhou, Xiaoyang [1 ]
Li, Niya [1 ]
Zhang, Decai [1 ]
Lu, Yanjun [1 ]
Wang, Ruigang [1 ]
Sun, Jian [1 ]
Lu, Cunfu [1 ]
Zheng, Xiaojiang [2 ]
Fritz, Eberhard [3 ]
Huettermann, Aloys [3 ]
Chen, Shaoliang [1 ,2 ]
机构
[1] Beijing Forestry Univ, Natl Engn Lab Tree Breeding, Coll Biol Sci & Technol, Beijing 100083, Peoples R China
[2] Hubei Univ Nationalities, Key Lab Biol Resources Protect & Utilizat Hubei P, Enshi 445000, Peoples R China
[3] Univ Gottingen, Busgen Inst, D-37077 Gottingen, Germany
来源
TREES-STRUCTURE AND FUNCTION | 2010年 / 24卷 / 04期
基金
中国国家自然科学基金;
关键词
ATPase; NaCl; Populus euphratica; Populus popularis; Respiratory rate; Salt tolerance; Western blotting; X-ray microanalysis; PLASMA-MEMBRANE H+; PUTATIVE NA+/H+ ANTIPORTER; POPULUS-EUPHRATICA; TONOPLAST VESICLES; ION FLUXES; TRANSPORT; SALINITY; STRESS; SODIUM; COMPARTMENTATION;
D O I
10.1007/s00468-010-0430-0
中图分类号
S7 [林业];
学科分类号
0829 ; 0907 ;
摘要
During a 30-day period of increasing salinity, we examined the effects of NaCl on leaf H+-ATPase and salinity tolerance in 1-year-old plants of Populus euphratica Oliv. (salt resistant) and P. popularis 35-44 (P. popularis) (salt sensitive). Electron probe X-ray microanalysis of leaf mesophyll revealed that P. euphratica had a higher ability to retain lower NaCl concentrations in the cytoplasm, as compared to P. popularis. The sustained activity of H+ pumps (by cytochemical staining) in salinised P. euphratica suggests a role in energising salt transport through the plasma membrane (PM) and tonoplast. Salt-induced alterations of leaf respiration, ATP content and expression of PM H+-ATPase were compared between the two species. Results show that P. euphratica retained a constant respiratory rate, ATP production and protein abundance of PM H+-ATPase (by Western blotting) in salt-stressed plants. P. euphratica was able to maintain a comparatively high capacity of ATP hydrolysis and H+ pumping during prolonged salt exposure. By contrast, the activity and expression of PM H+-ATPase were markedly decreased in P. popularis leaves in response to salt stress. Furthermore, NaCl-stressed P. popularis plants showed a marked decline of respiration (70%) and ATP production (66%) on day 30. We conclude that the inability of P. popularis to transport salt to the apoplast and vacuole was partly due to the decreased activity of H+ pumps. As a consequence, cytosolic ion concentrations were observed to be comparatively high for an extended period of time, so that cell metabolism, in particular respiration, was disrupted in P. popularis leaves.
引用
收藏
页码:597 / 607
页数:11
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