Rapid assay for amidohydrolase (urease) activity in environmental samples

被引：178

作者：

Sinsabaugh, RL ^{[1
]}

Reynolds, H

Long, TM

机构：

[1] Univ Toledo, Dept Biol, Toledo, OH 43606 USA

[2] Michigan State Univ, WK Kellogg Biol Stn, Hickory Corners, MI 49060 USA

来源：

SOIL BIOLOGY & BIOCHEMISTRY | 2000年 / 32卷 / 14期

关键词：

amidohydrolase; urease; soil enzyme assay; assay method; microplate assay;

D O I：

10.1016/S0038-0717(00)00102-4

中图分类号：

S15 [土壤学];

学科分类号：

0903 ; 090301 ;

摘要：

The use of microplate technology for enzyme assays has made it economical to measure a wide range of activities in environmental samples. Urease is one of the most widely measured soil enzyme activities, but current methods are cumbersome. We have developed a rapid, safe and sensitive assay that can be performed on microplates. (C) 2000 Elsevier Science Ltd. All rights reserved.

引用

页码：2095 / 2097

页数：3

共 14 条

[1]

Allen AS, 2000, ECOL APPL, V10, P437, DOI 10.1890/1051-0761(2000)010[0437:EOFACE]2.0.CO

[2]

[3]

BREMNER JM, 1978, SOIL ENZYMES, P149

[4] The marine cyanobacterium Synechococcus sp. WH7805 requires urease (urea amidohydrolase, EC 3.5.1.5) to utilize urea as a nitrogen source:: molecular-genetic and biochemical analysis of the enzyme [J].