Formation of a preinitiation complex by S-phase cyclin CDK-dependent loading of Cdc45p onto chromatin

被引：272
作者：
Zou, L
Stillman, B
机构：
[1] Cold Spring Harbor Lab, Cold Spring Harbor, NY 11724 USA
[2] SUNY Stony Brook, Grad Program Mol Genet & Microbiol, Stony Brook, NY 11794 USA
来源：
SCIENCE | 1998年 / 280卷 / 5363期
关键词：
D O I：
10.1126/science.280.5363.593
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Cdc45p, a protein essential for initiation of DNA replication, associates with chromatin after "start" in late G(1) and during the S phase of the cell cycle. Binding of Cdc45p to chromatin depends on Clb-Cdc28 kinase activity as well as functional Cdc6p and Mcm2p, which suggests that Cdc45p associates with the prereplication complex after activation of S-phase cyclin-dependent kinases (CDKs). As indicated by the timing and the CDK dependence, binding of Cdc45p to chromatin is crucial for commitment to initiation of DNA replication, During S phase, Cdc45p physically interacts with minichromosome maintenance (MCM) proteins on chromatin; however, dissociation of Cdc45p from chromatin is slower than that of MCMs, which indicates that the proteins are released by different mechanisms.
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页码：593 / 596
页数：4
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