Biomolecules under mechanical force

被引:188
作者
Kumar, Sanjay [1 ]
Li, Mai Suan [2 ]
机构
[1] Banaras Hindu Univ, Dept Phys, Varanasi 221005, Uttar Pradesh, India
[2] Polish Acad Sci, Inst Phys, PL-02668 Warsaw, Poland
来源
PHYSICS REPORTS-REVIEW SECTION OF PHYSICS LETTERS | 2010年 / 486卷 / 1-2期
关键词
Single-molecule force spectroscopy; DNA; Protein; Computer simulations; STEERED MOLECULAR-DYNAMICS; SINGLE-STRANDED-DNA; TITIN IMMUNOGLOBULIN DOMAINS; SEMIFLEXIBLE POLYMER-CHAIN; TRANSITION-STATE; UNFOLDING PATHWAYS; STATISTICAL-MECHANICS; FOLDING PATHWAY; DOUBLE HELIX; OVERSTRETCHING TRANSITION;
D O I
10.1016/j.physrep.2009.11.001
中图分类号
O4 [物理学];
学科分类号
0702 ;
摘要
Recent advances in single molecule experiments have raised many challenges. These challenges can be met by a proper understanding of the inter- and intra-molecular interactions in the framework of physics followed by suitable theoretical models substantiated by extensive numerical simulations. In this review, we briefly discuss experimental, theoretical and numerical techniques used to examine the dynamics of biomolecules under the application of external mechanical force. We focus on issues which require special attention: the relationship between the mechanical stability of a protein and the secondary structure of its native conformation, the dependence of the free energy landscape on the pulling direction and secondary structure content, unfolding of a protein through a pore, protein re-folding under quenched force etc. We pay special attention to a single stranded DNA, where the force-extension curve shows a multi-step plateau for the chain made up of adenine while poly-thymine exhibits entropic response only. There are many interesting predictions related to unzipping of double stranded DNA e.g. re-entrance in the force-temperature phase diagram, existence of an "Eye-phase", effects of random disorder etc. based on model studies which require further investigation. We will also discuss cases where the theoretical descriptions of the models fail to explain the experimentally observed behavior and when further refinement is needed in order to describe the outcomes of experiments. Finally we suggest certain experimental protocols to observe theoretical predictions in vitro. (C) 2009 Elsevier B.V. All rights reserved.
引用
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页码:1 / 74
页数:74
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