Evaluating the Effect of Lignocellulose-Derived Microbial Inhibitors on the Growth and Lactic Acid Production by Bacillus coagulans Azu-10

被引：19

作者：

Abdel-Rahman, Mohamed Ali ^{[1
,2
]}

Hassan, Saad El-Din ^{[1
]}

Fouda, Amr ^{[1
]}

Radwan, Ahmed A. ^{[1
]}

Barghoth, Mohammed G. ^{[1
]}

Desouky, Salha G. ^{[3
]}

机构：

[1] Al Azhar Univ, Dept Bot & Microbiol, Fac Sci, Cairo 11651, Egypt

[2] Al Azhar Univ, Al Azhar Ctr Fermentat Biotechnol & Appl Microbio, Cairo 11651, Egypt

[3] Suez Univ, Dept Bot & Microbiol, Fac Sci, Suez 41522, Egypt

来源：

FERMENTATION-BASEL | 2021年 / 7卷 / 01期

关键词：

lactic acid; lignocellulose-inhibitors; furans; Bacillus coagulans; xylose; thermophilic fermentation;

D O I：

10.3390/fermentation7010017

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Effective lactic acid (LA) production from lignocellulosic biomass materials is challenged by several limitations related to pentose sugar utilization, inhibitory compounds, and/or fermentation conditions. In this study, a newly isolated Bacillus coagulans strain Azu-10 was obtained and showed homofermentative LA production from xylose with optimal fermentation conditions at 50 degrees C and pH 7.0. Growth of strain Azu-10 and LA-fermentation efficiency were evaluated in the presence of various lignocellulose-derived inhibitors (furans, carboxylic acids, and phenols) at different concentrations. Furanic lignocellulosic-derived inhibitors were completely detoxified. The strain has exhibited high biomass, complete xylose consumption, and high LA production in the presence of 1.0-4.0 g/L furfural and 1.0-5.0 g/L of hydroxymethyl furfural, separately. Moreover, strain Azu-10 exhibited high LA production in the presence of 5.0-15.0 g/L acetic acid, 5.0 g/L of formic acid, and up to 7.0 g/L of levulinic acid, separately. Besides, for phenolic compounds, p-coumaric acid was most toxic at 1.0 g/L, while syringaldehyde or p-hydroxybenzaldehyde, and vanillin at 1.0 g/L did not inhibit LA fermentation. The present study provides an interesting potential candidate for the thermophilic LA fermentation from lignocellulose-derived substrates at the industrial biorefinery level.

引用

页数：16

共 42 条

[1]

Abdel-Rahman M. A., 2019, BIORESOUR TECHNOL RE, V7, DOI DOI 10.1016/J.BITEB.2019.100256

[2] Opportunities to overcome the current limitations and challenges for efficient microbial production of optically pure lactic acid [J].