Analysis of translocations that involve the NUP98 gene in patients with 11p15 chromosomal rearrangement

被引：30
作者：
Kobzev, YN
Martinez-Climent, J
Lee, S
Chen, JJ
Rowley, JD
机构：
[1] Univ Chicago, Med Ctr, Dept Med, Hematol Oncol Sect,Biol Sci Div, Chicago, IL 60637 USA
[2] Univ Valencia, Sect Mol Cytogenet Canc, Hosp Clin, Valencia, Spain
来源：
GENES CHROMOSOMES & CANCER | 2004年 / 41卷 / 04期
关键词：
D O I：
10.1002/gcc.20092
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
The NUP98 gene has been reported to be fused with at least 15 partner genes in leukemias with 11p15 translocations. We report the results of screening of cases with cytogenetically documented rearrangements of 11p15 and the subsequent identification of involvement of NUP98 and its partner genes. We identified 49 samples from 46 hematology patients with 11p15 (including a few with 11p14) abnormalities, and using fluorescence in situ hybridization (FISH), we found that NUP98 was disrupted in 7 cases. With the use of gene-specific FISH probes, in 6 cases, we identified the partner genes, which were PRRXI (PMXI; in 2 cases), HOXD13, RAP1GDS1, HOXC13, and TOP1. In the 3 cases for which RNA was available, RT-PCR was performed, which confirmed the FISH results and identified the location of the breakpoints in patient cDNA. Our data confirm the previous findings that NUP98 is a recurrent target in various types of leukemia. (C) 2004 Wiley-Liss, Inc.
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页码：339 / 352
页数：14
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